PEP-3与HBc融合原核表达载体的构建与表达  

The construction and expression of the prokaryotic expression vector of HBc-VLPs displaying PEP-3 peptide

作　　者：杨星[1] 陈皓[1] 叶明付 代鑫[1] 夏海滨[1] 

机构地区：[1]陕西师范大学生命科学学院秦巴山区可持续发展协同创新中心,陕西西安710119

出　　处：《陕西师范大学学报（自然科学版）》2015年第3期68-73,共6页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(81272543;81301957;81471772);中央高校基本科研业务费专项资金项目(GK201301010);陕西省自然科学基金重点项目(2014JZ005)

摘　　要：以可诱导较强免疫反应及呈现高拷贝抗原小肽的乙肝病毒核心蛋白(Hepatitis B Virus Core Protein,HBc)类病毒颗粒(Virus-like Particles,VLPs)作为免疫载体蛋白,通过分子生物学手段,将蓝白班筛选元件Lac Zα插入HBc的主要免疫显性区域,构建获得外源小肽与HBc融合的通用原核表达载体。在此通用载体的基础上,通过PEP-3小肽替换Lac Zα片段,构建了PEP-3与HBc融合原核表达载体,并成功进行了HBc/PEP-3融合蛋白的表达及纯化。该新型通用载体的成功构建,将为小肽疫苗的研究及应用提供有效工具。Hepatitis B virus core protein like particles(HBc-VLPs),which have a strong immune response and present the high copy number of antigen epitope,were used as epitope carriers.By taking methods of molecular biology,a white/blue screening element Lac Zαwas placed into the major immunodominant region of HBc-VLPs,and the prokaryotic expression vector for HBc con-taining the epitope peptide was constructed.Based on the vector,the prokaryotic expression vec-tor of HBc/PEP-3 was constructed by replacing Lac Zαelement with PEP-3 peptide consisting of 1 3 amino acids,then the expression and purification of fusion protein HBc/PEP-3 were success-fully carried out.The construction of the new universal vector will provide an efficient tool for the research and application of peptide vaccine.

关 键 词：乙肝病毒核心蛋白(HBc) 原核表达载体 类病毒颗粒 肽疫苗 

分 类 号：Q78[生物学—分子生物学] R373.21[医药卫生—病原生物学]