子宫直肠陷凹子宫内膜异位症对Wistar大鼠生殖能力影响的研究  

Influence of Uterine Rectal Pouch Endometriosis on Reproductivity of Wistar Rats

作　　者：张薇[1] 黎福荣[1] 唐彬[1] 赵勇[1] 林以理[1] 曾维树[1] 

机构地区：[1]中山大学实验动物中心,广州510080

出　　处：《实验动物科学》2015年第3期19-22,53,共5页Laboratory Animal Science

基　　金：广东省科技基础条件建设项目(No:2012B060300015)

摘　　要：目的构建Wistar大鼠子宫直肠陷凹内膜异位症模型,对其病灶病理学形态及受孕率进行统计。方法采用异体移植方法在40只Wistar大鼠的子宫直肠凹陷处移植子宫组织,建立子宫直肠陷凹子宫内膜异位症动物模型,10只行假手术,8周后投放雄鼠,观察雌鼠的产仔率,于第15周取材剥取模型大鼠在位与异位子宫内膜组织,进行组织学观察。结果组织学上异位内膜的细胞形态呈增殖早期到分泌中期改变,和在位内膜呈增殖早期到分泌晚期改变发育不同步;模型组大鼠平均产仔数为6.02只,显著较假手术组8只及对照组为8.5只低(P<0.01)。结论 Wistar大鼠子宫内膜异位症内膜与在位内膜发育不同步,造成大鼠腹腔内环境异常,使受精卵着床变得更加困难,从而降低了大鼠的生殖能力。Objective To establish the uterine rectal pouch endometriosis model using female Wistar rat,and study the histological structure and pregnancy rates in those rats. Method Autologous uterus were transplanted into 40 female Wistar rats to establish the uterine rectal pouch endometriosis model,and 10 rats were treated as the shamoperated control. Male rats were permitted to approach the female models or controls 8 weeks after transplantation,and the farrowing rates and litter sizes were detected. The eutopic and ectopic endometrium were collected 15 weeks after transplantation for histological analysis. Result Based on the histological examination,development of the ectopic endometrium is different from that of the eutopic endometrium. The cell morphology of the ectopic endometrium changed from early proliferative phase to mid-secretory phase,but that of the eutopic endometrium changed from early proliferative phase to later secretory phase. The average litter size of model group was 6. 02,which was significant lower than the control group( 8. 5)( P < 0. 01) or the sham operated group( 8. 0)( P <0. 01). Conclusion Development of the ectopic endometrium is not the same as that of the eutopic one. It makes the rat body environment abnormal,implantation of fertilized eggs difficult,and so reduces the reproductiveity.

关 键 词：子宫直肠陷凹 子宫内膜异位症 动物模型 移植 产仔率 

分 类 号：R711.71[医药卫生—妇产科学]