果糖二磷酸钠镁对缺血突触体乳酸含量及ATP酶活性的影响(英文)  

Effects of sodium magnesium fructose diphosphate on lactate concentration and ATPase activity of ischemic synaptosome

作　　者：曾凡新[1] 董志[2] 周岐新[2] 

机构地区：[1]重庆医药工业研究院药理毒理室,重庆400061 [2]重庆医科大学药理教研室,重庆400016

出　　处：《中国临床药理学与治疗学》2002年第6期493-495,共3页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的 :研究果糖二磷酸钠镁 (FDPM)对缺血突触体乳酸含量及ATP酶活性的影响 ,以探讨其脑保护作用机制。方法 :制备正常大鼠脑突触体 ,氧糖剥夺培养建立缺血突触体模型 ,分别加 1.3mmol·L- 1硫酸镁 ,4 .0mmol·L- 11,6 二磷酸果糖 (FDP)及 1.3mmol·L- 1果糖二磷酸钠镁共培养 6 0min ,测定突触体乳酸含量及Na+ K+ATP酶和Ca2 + Mg2 +ATP酶活性。结果 :FDPM可明显降低缺血突触体乳酸含量 ,升高Na+ K+ATP酶和Ca2 + Mg2 +ATP酶活性(P <0 .0 5 ) ,其作用强于FDP的作用。结论 :FDPM保护脑缺血的作用机制可能与其改善脑缺血后能量代谢 。AIM: To study the effects of sodium magnesium fructose diphosphate (FDPM) on lactate concentration and ATPase activity of ischemic synaptosome, so as to explore the protective mechanisms of FDPM on cerebral ischemia. METHODS: Synaptosome prepared from normal rat brain was cultured with oxygen and glucose deprived to establish the model of ischemic synaptosome. 1.3 mmol·L -1 magnesium sulfate, 4.0 mmol·L -1 fructose 1,6 diphosphate (FDP) and 1.3 mmol·L -1 FDPM were incubated with the ischemic synaptosomes separately. After 60 min, the lactate concentration as well as the activities of Na + K + ATPase and Ca 2+ Mg 2+ ATPase were detected. RESULTS: FDPM reduced the lactate concentration, raised the activities of Na + K + ATPase and Ca 2+ Mg 2+ ATPase of ischemic synaptosomes (P< 0.05 ). The effects of FDPM were stronger than those of FDP (P< 0.05 ). CONCLUSION: FDPM may protect cerebral ischemia by improving the energy metabolism, and mitigating acidosis state of brain tissue.

关 键 词：脑缺血 果糖二磷酸钠镁 1 6-二磷酸果糖 硫酸镁 突触体 乳酸 NA^+-K^+ATP酶 Ca^2+-Mg^2+ATP酶 

分 类 号：R963[医药卫生—微生物与生化药学]