阻断血管内皮细胞乙酰胆碱靶标功能对血管内皮细胞结构和功能的影响  

Effects of anisodamine on vascular endothelium through blocking endothelial target for acetylcholine

作　　者：陈凯[1] 张雁芳[1] 汪海[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《中国临床药理学与治疗学》2003年第5期511-514,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家重大基础研究发展规划项目(№G19980 51112)

摘　　要：目的 :研究阻断血管内皮细胞乙酰胆碱靶标(ETA)功能对血管内皮细胞结构和功能的影响。方法 :以山莨菪碱为工具药 ,喂食兔 3mon后急性处死 ,剖腹取主动脉 ,HE及ET +VG染色光镜观察主动脉的组织学改变 ,电镜观察血管内皮细胞的超微结构改变。同时取胸主动脉、肺动脉、颈动脉、肾动脉和股动脉 ,进行离体血管功能实验。结果 :生理状态下 ,长期给予兔山莨菪碱 ,血管内皮细胞结构受损 ,光镜下可见内皮细胞垂直附着在内弹力板上 ,电镜下可见内皮细胞呈网眼状损伤 ,有些内皮细胞即将脱落 ,线粒体肿胀。离体血管环实验研究发现ETA介导的内皮依赖性血管舒张反应显著减弱。结论 :生理状态下 ,反复阻断ETA ,可使内皮细胞结构和功能受损 。AIM: To investigate the change of endothelial structure and function by blocking endothelial target for acetylcholine with anisodamine. METHODS: Rabbits were killed after having been fed with anisodamine for three months. The morphological changes of vascular walls of aorta were observed through optical macroscope, and the morphological changes of vascular endothelial cells under electronic microscopes. The functions of aorta, pulmonary, carotid, renal and femoral vascular were measured by isolated vascular ring experiment. RESULTS: The aorta endothelial morphology of rabbits changed in treated with anisodamine for three months. The endothelial cells vertically adhered to internal elastic lamina. Some endothelial cells were mesh shaped changed and some were liable to desquamate. The endothelium-dependent vascular relaxation induced by acetylcholine was attenuated significantly. CONCLUSION: The vascular endothelial structure and function is damaged by blocking endothelial target for acetylcholine.

关 键 词：药理学 血管内皮细胞乙酰胆碱靶标 内皮细胞 山莨菪碱 乙酰胆碱 舒张反应 

分 类 号：R965[医药卫生—药理学] R971.92[医药卫生—药学]