银杏外种皮多糖对SGC-7901细胞p53基因的表达及端粒酶活性的影响  

Effect of ginkgo biloba exocarp polysaccharides (GBEP) on p53 gene expression and telomerase activity of human gastriccancer SGC-7901 cells

作　　者：许爱华[1] 陈华圣[2] 陈钢[3] 苏庆[4] 孙步蟾[5] 顾玉兰[5] 

机构地区：[1]扬州大学医学院药理学教研室,扬州225001 [2]扬州大学医学院方药教研室,扬州225001 [3]扬州大学医学院病理学教研室,扬州225001 [4]扬州大学医学院形态学实验室,扬州225001 [5]扬州大学医学院医学系,扬州225001

出　　处：《中国药理学通报》2003年第10期1174-1176,共3页Chinese Pharmacological Bulletin

基　　金：江苏省社会发展科技计划基金资助 NoBS2 0 0 0 0 86;江苏省教育厅自然科学基金资助 No 99KJD3 60 0 0 1

摘　　要：目的　研究银杏外种皮多糖对人胃癌SGC 790 1细胞 p5 3基因表达及端粒酶活性的影响。 方法　应用免疫组织化学ABC法检测 p5 3基因的表达 ;应用TRAP ELISA法检测端粒酶活性。结果　银杏外种皮多糖 (80～ 16 0mg·L-1,4 8h)能抑制人胃癌SGC 790 1细胞突变型 p5 3基因的表达及其端粒酶活性。结论　银杏外种皮多糖抑制SGC 790 1细胞增殖的作用机制可能与其下调突变型 p5AIM To study the influence of ginkgo biloba exocarp polysaccharides(GBEP) on p53 gene expression and telomerase activity of human gastriccancer SGC 7901 cells in vitro . METHODS The p53 gene expression level of human gastriccancer SGC 7901 cells were measured by immunohistochemistry ABC. The telomerase activity of human gastriccancer SGC 7901 cells were measured by TRAP ELISA. RESULTS GBEP(80～160 mg·L -1 ,48 h) inhibited the expression of mutated type p53 genes and telomerase activity of human gastriccancer SGC 7901 cells. CONCLUSION The action mechanism of GBEP that inhibited the proliferation of SGC 7901 cells may relate to its down regulation effect on the expression of p53 genes and telomerase activity of human gastriccancer SGC 7901 cells.

关 键 词：银杏 多糖 胃癌细胞 P53基因 端粒酶 

分 类 号：R282.71[医药卫生—中药学] R284.1[医药卫生—中医学]