妊高征母儿内皮型一氧化氮合酶基因多态性的检测  

Detection of Endothelial Nitric Oxide Synthase Gene Polymorphism in Preeclampsia

作　　者：周容[1] 杨元[2] 熊庆[1] 曹西蓉[3] 

机构地区：[1]四川大学华西第二医院妇产科,成都610041 [2]四川大学华西医院医学遗传室 [3]四川大学华西公共卫生学院环境卫生学教研室

出　　处：《四川大学学报（医学版）》2003年第4期671-673,共3页Journal of Sichuan University(Medical Sciences)

基　　金：四川大学校基金 (编号 L 9915 )资助

摘　　要：目的　了解内皮型一氧化氮合酶 (e NOS)基因多态性与妊高征的关系。方法　采用聚合酶链反应、高分辨琼脂糖电泳与 DNA测序技术检测 31对母儿 (妊高征 15对 ,正常妊娠妇女 16对 ) e NOS基因内含子 4多态性。结果　 6 2个标本中出现 3种等位基因 ,即 b(4 2 0 bp)、a(393bp)和 c(4 4 7bp) ,其出现频率分别为 91.93%、 6 .4 5 %和 1.6 1%。 6 2个标本中出现 3种基因型 ,即 b/ b、a/ b和 c/ b,其出现频率分别为 83.83%、12 .90 %和 3.2 7% ;妊高征妇女和正常妊娠妇女、妊高征妇女的新生儿及正常妊娠妇女的新生儿以及妊高征母儿间 e NOS基因内含子 4基因频率与基因型频率均无显著差异。结论　 e NOS基因内含子Objective To detect the relationship between endothelial nitric oxide synthase gene polymorphism and preeclampsia. Methods The study included the samples from 62 subjects(15 preeclampsia pregnant women with their infants and 16 normal pregnant women with their infants). Screening for the endothelial nitric oxide synthase gene polymorphism, the 27 bp repeat in intron 4(eNOS4),was carried out by analysis of polymerase chain reaction and DNA sequencing. Results The frequency of the b allele (420 bp) in the 62 samples was 91.93%, the frequency of the a allele (393 bp) was 6.45%, and that of the c allele (447bp) was 1.61%. The frequencies of eNOS 4 b/b, eNOS 4 a/b and eNOS4 c/b were 83.83%, 12.90% and 3.27%, respectively. There was no significant difference of eNOS4 polymorphism between the preeclampsia pregnant women and the normal pregnant women, between their infants, and between the preeclampsia pregnant women and their infants. Conclusion The eNOS 4 polymorphism may be not associated with preeclampsia. Further studies are needed to confirm the eNOS gene polymorphism with preeclampsia.

关 键 词：基因多态性 内皮型一氧化氮合酶 心血管并发症 妊高征 

分 类 号：R714.246[医药卫生—妇产科学] R394[医药卫生—临床医学]