2,4-二氯苯酚低浓度长期暴露对鲫鱼肝脏抗氧化系统的影响  

Effects of Long-term Exposure of Low-level 2,4-Dichlorophenol on the Antioxidant Defense System in Liver of Carassius auratus

作　　者：张景飞[1] 王晓蓉[1] 

机构地区：[1]南京大学环境学院污染控制与资源化研究国家重点实验室,南京210093

出　　处：《环境科学》2003年第5期136-140,共5页Environmental Science

基　　金：国家教育部高等学校博士点专项基金资助课题(2 0 0 0 0 2 842 4)

摘　　要：在室内模拟条件下 ,研究了低浓度 2 ,4 二氯苯酚 (2 ,4 DCP)长期暴露 (4 0d)对鲫鱼 (Carassiusauratus)幼体肝脏抗氧化系统的影响 .结果表明 :过氧化氢酶 (CAT)活性、谷胱甘肽过氧化物酶 (Se GPx)活性、氧化型谷胱甘肽(GSSG)含量可被显著诱导 ;超氧化物歧化酶 (SOD)活性在 0 0 0 5mg·L- 1　 2 ,4 DCP污染胁迫下即被显著诱导 ;还原型谷胱甘肽 (GSH)含量几乎持续受到抑制 ;谷胱甘肽还原酶 (GR)活性则先受到抑制后逐渐回升 ;谷胱甘肽硫转移酶 (GST)的活性变化较小 ,仅在 2个低浓度组中有轻微诱导 .GR、GSH ,尤其是SOD ,对 2 ,4 DCP较为敏感 ,可以作为水生生态系统中 2 ,4 DCP污染的一项早期监测指标 .Under the indoors simulant conditions, the effects of long-term exposure of low-level 2,4-Dichlorophenol on the antioxidant system of larval fishes were studied. The results showed that the activities of catalase (CAT), sedium-dependent glutathione peroxidase (Se-GPx) and the content of oxidized glutathione (GSSG) were induced significantly. The activity of superoxide dismutase (SOD) was induced remarkably when 2,4-Dichlorophenol level was at only 0.005 mg·L -1; the content of reduced glutathione (GSH) was almost suppressed continuously; the activity of glutathione reductase was inhibited firstly and then restored gradually; the activity of glutathione S-transferase (GST) had slight variances among groups. GR、GSH,especially SOD, can act as an early monitoring index of 2,4-Dichlorophenol pollution in aquatic ecosystem.

关 键 词：鲫鱼 2 4-二氯苯酚 抗氧化防御系统 酶活性测定 

分 类 号：R994.6[医药卫生—毒理学]