mecA、femA基因PCR联合扩增法检测耐甲氧西林金黄色葡萄球菌  

Combination PCR of mecA, femA Genes for Detection of MRSA

作　　者：陈智鸿[1] 范昕建[1] 吕晓菊[1] 

机构地区：[1]四川大学华西医院传染科,成都610041

出　　处：《四川大学学报（医学版）》2003年第4期663-666,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金 (批准号 3 9970 675 )资助

摘　　要：目的　探讨 m ec A、fem A基因 PCR联合扩增快速检测耐甲氧西林金黄色葡萄球菌 (MRSA )的敏感性及特异性。方法　用纸片扩散法及 m ec A、fem A基因 PCR联合扩增检测 178株葡萄球菌中的 MRSA,并与琼脂稀释法的结果比较。结果　纸片扩散法筛检 MRSA (耐甲氧西林凝固酶阴性葡萄球菌 ,MRCNS)的敏感性、特异性分别为 94 .4 % (89.5 % )、92 .4 % (73.7% )。在金黄色葡萄球菌中若以 mec A基因阳性为判定 MRSA的指标 ,则敏感性、特异性分别为 91.7%、95 .5 %。在 178株葡萄球菌中若以 mec A、fem A基因阳性作为判定 MRSA的指标 ,特异性提高到 97.9%。结论　 PCR m ec A、fem A基因联合扩增既可用于筛检 MRSA,又可免去常规生化鉴定 ,具快速。Objective To make a comparison of PCR assay, Agar Dilution and Disk Diffusion as for detecting Staphylococcal mecA, femA genes. Methods A total of 178 strains of Staphylococci were isolated from three large scale hospitals in Chengdu. Disk Diffusion and staphylococcal mecA, femA gene PCR assay for detecting MRSA were compared with Agar Dilution. Results The sensitivity and specificity of Disk Diffusion for detecting MRSA were 94.4% and 92.4% respectively. The sensitivity and specificity of Disk Diffusion for detecting MRCNS were 89.5% and 73.7% respectively. Compared with Agar Dilution, Staphylococcus aureus mecA gene PCR assay's sensitivity and specificity were 91.7% and 95.5% respectively. In all the 178 strains of Staphylococci, mecA gene combining with femA gene PCR assay's specificity was up to 97.9%. Conclusion The detection of femA gene together with mecA gene by PCR is not only an approach for differentiating MRSA from MRCNS, but also a rapid, highly specific method which can be used as an auxiliary method in clinical microbiological laboratory.

关 键 词：耐甲氧西林金黄色葡萄球菌 耐甲氧西林凝固酶阴性葡萄球菌 mecA基因 FEMA基因 PCR 

分 类 号：R446.5[医药卫生—诊断学]