增液汤对地塞米松诱导幼鼠胸腺细胞凋亡的影响  

Effects of Zengyetang Injection on Apoptosis of Thymocytes Induced by Dexamethasone in Young Rats

作　　者：王君[1] 仝小林[1] 杨凌[1] 王坤[2] 迟永春[2] 叶智文[2] 杨梦兰[1] 

机构地区：[1]中日友好医院儿科,北京100029 [2]中日友好医院临床医学研究所病理生理室,北京100029

出　　处：《北京中医药大学学报》2003年第5期35-38,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助课题 (No .3 9670 866)

摘　　要：目的　观察增液汤注射剂对地塞米松诱导幼鼠胸腺细胞凋亡的调节作用。方法　给 4～ 5周龄Wistar大鼠腹腔注射增液汤注射剂和地塞米松 ,采用DNA琼脂糖凝胶电泳、流式细胞光度计分析不同处理组的胸腺凋亡细胞。同时电镜观察各组胸腺细胞的超微结构变化。结果　DNA琼脂糖凝胶电泳显示增液汤组仅有微弱的梯形条带。流式细胞光度分析地塞米松组DNA组方图上出现一个较大的凋亡峰 ,其凋亡细胞百分率为 (6 0 74± 4 86 ) % ;而增液汤组的二倍体核型峰较地塞米松组明显高大 ,凋亡峰却明显缩小 ,其凋亡细胞百分率约为 (32 2 0± 7 70 ) % ,与地塞米松组相比 ,显著差异 (P <0 0 1)。电镜观察发现 ,地塞米松组细胞染色质凝集呈新月状分布 ,或形成凋亡小体 ;增液汤组细胞形态变化不显著 ,核染色质均匀 ,仅有体积轻微缩小。Objective To observe the effects of Zengyetang Injection (ZI) on apoptosis of thymocytes induced by dexamethasone in young rats. Methods Wistar rats aged 4～5 weeks were given ZI and dexamethasone by intraperitoneal injection. DNA agarose gel electrophoresis and flow cytometry were used to analyse apoptosis of thymocytes in both the ZI group and the dexamethasone group. The electron microscope was used to observe the changes in the ultrastructure of thymocytes in both groups. Results DNA agarose gel electrophoresis showed that there was only a weak ladder strip in the ZI group. Flow cytometry showed that there was a rather big apoptotic peak in the DNA group block diagram, with an apoptotic rate of (60 74±4 86)% in the dexamethasone group; the diplokaryon peak was obviously bigger and the apoptotic peak was obviously smaller in the ZI group as compared with those in the the dexamethasone group; the apoptotic rate in the ZI group was (32 20±7 70)%. There was a significant difference between the two apoptotic rates ( P <0 01). The observation by electron microscope showed that the chromatin of thymocytes gathered in a crescent shape or formed apoptotic corpuscles in the dexamethasone group while in the ZI group, the chromatin existed in an even status and only the volume shrunk slightly. Conclusion ZI has certain inhibitory effects on the apoptosis of thymocytes induced by dexamethasone.

关 键 词：增液汤 肾上腺皮质激素类 细胞凋亡 幼鼠 

分 类 号：R285.5[医药卫生—中药学]