RP-HPLC和TLCS测定黄连解毒汤有效部位中藏红花酸含量的方法研究  

Methodological Study of the Quantitative Determination of Crocetin in the Active Fraction of Huanglianjiedu Decoction by RP-HPLC and TLCS

作　　者：赵文华[1] 石任兵[1] 刘斌[1] 周菲[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《北京中医药大学学报》2003年第5期63-66,共4页Journal of Beijing University of Traditional Chinese Medicine

摘　　要：目的　建立黄连解毒汤有效部位中藏红花酸的含量测定方法。方法　采用高效液相色谱法(HPLC)及薄层色谱扫描法 (TLCS)。HPLC法 :YWG C18色谱柱 ;流动相 :甲醇—水—冰乙酸 ;检测波长 :4 2 3nm。薄层色谱扫描法 :硅胶G薄层板 ;展开剂 :氯仿—甲醇—甲酸 ;反射法锯齿扫描 ,入射波长 :4 10nm ;参比波长 :5 6 0nm。结果　HPLC法平均回收率为 10 1 6 8% ,RSD =1 16 % (n =5 ) ;TLCS法平均回收率为 98 5 4% ,RSD =0 83% (n =5 )。结论　两种方法测定结果基本一致 ,且操作简便、准确 ,均可作为黄连解毒汤有效部位的质量控制方法。Objective To establish a method for quantitatively determining the content of crocetin in the active fraction of Huanglianjiedu Decoction (HD). Methods Both HPLC and TLCS were used in the experiment. For HPLC, the YWG C 18 column was chosen, the mobile phase was methanol water glacial acetic acid, and the detection wavelength was 423 nm. For TLCS, the silica gel G plate was chosen, the developing agent was chloroform methanol formic acid, the scanning was carried out by reflection sawtooth scanning, the incident wavelength (λR) was 410 nm, and the reference wavelength (λS) was 560 nm. Results For HPLC, the average recovery was 101 68% and RSD was 1 16% ( n =5); and for TLCS, the average recovery was 98 54% and RSD was 0 83% ( n =5). Conclusion The results achieved by the two methods are basically the same. Both methods are simple and accurate and can be used in the quality control of the active fraction of HD.

关 键 词：黄连解毒汤 藏红花酸 高效液相色谱法 薄层色谱扫描法 含量测定 

分 类 号：R284.2[医药卫生—中药学]