染色单体互换频率与铅砷等危害的探讨  

A study on the effects of occupational exposure to lead,arsenic and cadmium on sister chromatid exchange rate in human lymphocytes

作　　者：陈晓琴[1] 陈婷婷[1] 江世强[1] 苏素花[1] 周少诗[1] 翟日洪[1] 甘永金[1] 梁启荣[1] 陈志强[1] 庞丽[1] 

机构地区：[1]广西职业病防治研究所,广西南宁530021

出　　处：《中国工业医学杂志》2003年第4期206-208,共3页Chinese Journal of Industrial Medicine

基　　金：广西科技厅自然科学基金课题 (991 2 0 30 )

摘　　要：目的　探讨铅、镉、砷等职业有害因素致人外周血淋巴细胞染色体畸变的特征性改变。方法　采集职业作业人群及正常人群外周血淋巴细胞培养 72h ,观察染色体畸变类型及其特征。结果　因镉污染接触人群及实验室条件下对淋巴细胞进行高浓度染铅时染色单体互换畸变细胞率为 0 ;染砷浓度 <0 1mg/L时未发生染色单体互换 ,染砷浓度达 0 2～ 0 4mg/L时染色单体互换畸变细胞率为 1 2 4%。　结论　染色单体互换畸变细胞率的增加与接触因素砷及砷的浓度密切相关 。Objective To investigate the effects of occupational exposure to lead,cadmium and arsenic on chromosome aberration(CA)in human lymphocytes.Method Lymphocytes extracted from the workers occupationally exposured to lead,cadmium and arsenic and normal healthy workers were cultured for 72 hours.Their chromosome aberration was observed and characterized under light microscope. Result Sister chromatid exchange rate in lymphocytes of the workers exposed to cadmium and in those experimentally exposed to high-level of lead,was zero.No sister chromatid exchange was observed in lymphocytes cultured under<0.1 mg/L arsenic concentration.Sister chromatid exchange rate of 1.24% was deteced in lymphocytes exposed to 0.2-0.4 mg/L of arsenic.Conclusion Sister chromatid exchange rate in lymphocytes was closely correlated to arsenic exposure levels.The role of exposure to arsenic in sister chromatid exchange needs further investigation.

关 键 词：染色单体互换 职业因素 外周血 淋巴细胞 细胞培养 砷污染 

分 类 号：R135.1[医药卫生—劳动卫生]