过度训练对人精子染色质结构的影响  

The effect of overtraining on human sperm chromatin structure

作　　者：丁晓萍[1] 闫素文[1] 张宁[1] 张黎[1] 汤洁[1] 鲁海鸥[1] 

机构地区：[1]解放军202医院全军优生优育研究中心,沈阳110003

出　　处：《中华劳动卫生职业病杂志》2003年第4期260-262,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：全军医药卫生科研"十五"基金项目重点课题资助项目 (0 1Z0 0 2 )

摘　　要：目的　探讨过度训练是否对人精子DNA产生影响。方法　应用流式细胞仪快速测量精子核原位DNA损伤和完整性 ,分析精子染色质结构 (SCSA) ,对两组不同作业环境 (过度训练组与非训练组 )的 2 4 9名男性官兵进行分子流行病学调查。结果　训练组SCSA中COMPατ的平均值为11.0 2 % ,而对照组仅为 5 .90 % ,两组比较差异有显著性 (P <0 .0 1)。COMPατ参数与精子活力密切相关 (r=0 .4 1,P <0 .0 5 )。结论　过度训练后可导致精子染色质结构的变化 ,影响精子活力 ,使生育潜能降低。Objective To identify the effects of overtraining on human sperm DNA. Methods Molecular epidemiological investigation of 249 men from different groups(training and non-training)was carried out by using flow cytometer to detect the integrity and damage of in situ DNA of sperm nucleus, and sperm chromatin structure assay was performed. Results The average COMPαt in training group was 11.02% while that in control group was 5.90%( P <0.01).COMPαt was significantly correlated with sperm activity( r =0.41, P <0.05). Conclusion Overtraining could induce sperm DNA injury and affect sperm activity,thus to decrease the potentiality of reproduction.

关 键 词：精子染色质结构分析 过度训练 精子活力 分子流行病学调查 

分 类 号：R82[医药卫生—临床医学]