脂质体携载金属硫蛋白对皮瓣继发静脉缺血再灌注损伤的作用  

The effect of liposome-carried metallothionein on secondary venous ischemia-reperfusion injury in a rat flap

作　　者：郭杰[1] 王冬艳[1] 关德宏[1] 唐朝枢[2] 

机构地区：[1]哈尔滨医科大学附属二院整形外科,150086 [2]哈尔滨北京大学医学生理学系

出　　处：《中华整形外科杂志》2003年第4期245-247,共3页Chinese Journal of Plastic Surgery

摘　　要：目的　观察脂质体包裹金属硫蛋白 (MT)对岛状皮瓣继发静脉缺血再灌注损伤的保护作用。方法　在大鼠下腹部岛状皮瓣继发静脉缺血再灌注损伤模型上测定皮瓣即刻、继发缺血再灌注 30min、7d后的丙二醛 (MDA)含量、髓过氧化物酶 (MPO)活性 ,继发缺血再灌注 30min时血浆内皮素 (ET)、乳酸脱氢酶 (LDH)水平 ,7d后皮瓣MT的含量。结果　脂质体携载MT组明显降低皮瓣MDA、MPO、血浆ET、LDH含量 ,增加 7d后皮瓣组织MT含量 ,提高皮瓣成活率。结论　脂质体包裹MT对皮瓣继发静脉缺血再灌注损伤具有良好的保护作用。Objective To investigate the effect of liposome-carried metallothionein (lipo-MT) on secondary ischemia-reperfusion injury in the rat island flap. Methods An abdominal island flap was created in the Wistar rat. The animals were divided into four groups: the sham group, the secondary ischemia-reperfusion group, the group treated with blank liposome and the group treated with lipo-MT. The malondialdehyde (MDA) content, the myeloperoxidase (MPO) activity was assayed immediately, at 30 minutes and 7 days after the secondary venous ischemia-reperfusion. The level of endothelin (ET) and lactic dehydrogenase (LDH) of the rat plasma was measured at 30 minutes after secondary venous ischemia-reperfusion. The content of MT of the flap was assayed by Cd-hemoglobin saturation method at 7 days after the operation. Results The treatment of lipo-MT significantly decreased the content of MDA, MPO of the flap, decreased the activity of ET, LDH of the rat palsma, increased the content of MT of the flap and improved the flap viability. Conclusion Lipo-MT can improve flap survival by reducing ischemia-reperfusion injury. ;

关 键 词：脂质体 金属硫蛋白 岛状皮瓣 缺血再灌注损伤 丙二醛 髓过氧化物酶 内皮素 乳酸脱氢酶 

分 类 号：R965[医药卫生—药理学]