靶向血管治疗增生性瘢痕的实验研究  

A research of endothelial cell-targeted therapy for cure of hypertrophic scar

作　　者：沈锐[1] 利天增[1] 祁少海[1] 梁惠珍[2] 徐盈斌[1] 谢举临[1] 吴义方[3] 冯祥生[4] 杜永军[4] 

机构地区：[1]中山医科大学第一附属医院,广州现在528000 [2]中山医科大学病理教研室 [3]中山医科大学电镜室 [4]广东佛山市第一人民医院烧伤整形科

出　　处：《中华整形外科杂志》2003年第4期254-257,共4页Chinese Journal of Plastic Surgery

基　　金：广东省科委基金资助 ( 98 -2 - 0 16 -0 1)

摘　　要：目的　探讨靶向血管治疗烧伤后增生性瘢痕的可行性和方法。方法　通过建立增生性瘢痕的裸鼠移植模型 ,使用不同剂量血管内皮生长因子 (VEGF)的单克隆抗体 ,进行瘢痕内注射治疗 ,观察瘢痕Ⅰ型、Ⅲ型胶原和血管的量以及其超微结构的变化。结果　靶向血管治疗 3周后 ,增生性瘢痕的体积明显减小 ,瘢痕胶原、血管明显减少 ,瘢痕内大量成纤维细胞坏死、凋亡 ,代之成熟的纤维细胞 ,失去了典型增生性瘢痕的特征。结论　血管内皮生长因子可能对增生性瘢痕中的大量血管新生起支持作用 ,大量血管对增生性瘢痕的胶原合成可能起着促进作用 ,针对VEGF的靶向血管治疗可能是治疗增生性瘢痕的的一种有意义的方法。Objective To investigate the feasibility of endothelial cell-targeted therapy to cure post-burn hypertrophic scar. Methods A hypertrophic scar animal model was made. Intralesional injecting of VEGF monoclonal antibody was performed for three weeks. The changes of scar in volume and morphology were observed. Results ① The volume of scar decreased.② The number of the capillary, the amount of collagen I and collagen III decreased. ③ Transmission electron microscope examinations demonstrated many dead or apoptotic fibroblasts and endothelial cells. Fibrocytes were seen relatively common. Conclusion VEGF induces the growth and development of hypertrophic scar in that it induces excessive and uncontrollable angiogenesis, which favors excessive collagen synthesis. Endothelial cell-targeted therapy may be a promising method to cure post-burn hypertrophic scar.;

关 键 词：增生性瘢痕 靶向血管治疗 血管内皮生长因子 瘢痕内注射治疗 血管新生 

分 类 号：R622[医药卫生—整形外科]