RT-PCR法测定牵拉性撕脱伤血管上P-选择素量的变化  

Change of expression of P-selectin in avulsion-injured vessels

作　　者：潘宝华[1] 李荟元[1] 鲁开化[1] 郭树忠[1] 夏炜[1] 卢丙仑[1] 武胜昔[2] 

机构地区：[1]第四军医大学西京医院整形外科中心,西安710032 [2]第四军医大学解剖教研室

出　　处：《中华整形外科杂志》2003年第4期288-290,共3页Chinese Journal of Plastic Surgery

基　　金：国家自然科学基金 ( 3 9470 70 5 )

摘　　要：目的　探讨牵拉性撕脱伤血管上的P 选择素的变化。方法　设定 6 0、70、80及 90g的牵拉力 ,利用血管损伤模型造成不同的血管损伤 ,用RT PCR法测定损伤血管上P 选择素量的变化。结果　撕脱伤血管上P 选择素mRNA表达随牵拉力增加而升高 ,静脉的表达要高于动脉。结论　结合以前的相关实验 ,提示P 选择素与血栓形成关系密切。Objective To determine the change of P-selectin in avulsion-injured vessels. Methods Different stretch forces of 60, 70, 80 and 90?g were applied to a vascular injury model.The expression changes of P-selectin were evaluated by RT-PCR. Results The expression of P-selectin mRNA in the injured vessels increased with the stretch force. Conclusion The result associated with our previous study indicated that P-selectin may be involved in thrombosis.;

关 键 词：牵拉性撕脱伤 血管损伤 P-选择素 血栓形成 反转录聚合酶链反应 

分 类 号：R641[医药卫生—外科学]