免疫组化方法检测猪与人皮肤通用抗原标志  

Determination of skin antigen marker of pig and human with Immunohistochemical method

作　　者：姜笃银[1] 张红[1] 周爱珍[1] 陈璧[2] 

机构地区：[1]江苏省泰州市创伤整形研究所泰州市第四人民医院烧伤整形外科,江苏泰州225300 [2]第四军医大学西京医院烧伤外科

出　　处：《中国冶金工业医学杂志》2003年第4期236-239,共4页Chinese Medical Journal of Metallurgical industry

基　　金：国家自然科学基金项目 (390 70 82 2 );泰州市海陵区科委专项资金资助

摘　　要：目的 :探讨猪与人皮肤组织蛋白抗原性的差异。方法 :人和小猪全厚正常皮肤做冰冻切片 ,用抗人皮肤组织相关抗原 (HLA -ABC ,HLA -DR ,Vimentin ,Desmin ,Actin ,FⅧRA ,Cytokeratin)、细胞因子及其受体和胞外基质 (Hyaluronicacid ,Fibronectin ,Laminin ,TypeⅣcollagen ,Estradiol- 1 7β和Tstosterone)及I、Ⅲ、Ⅶ型胶原等抗原成分的单克隆或多克隆抗体进行免疫组化染色 ,比较猪与人皮肤组织抗原性异同。 结果 :在猪皮肤组织可检测到Ⅰ、Ⅲ、Ⅳ、Ⅶ型胶原和Hyaluronicacid ,Fibronectin ,Laminin ,FⅧRA ,Cytokeratin ,IL - 6 ,IL - 8,Estradiol- 1 7β,Tstosterone和ICAM - 1 ;多克隆抗体阳检率明显高于单克隆抗体 ;其中细胞外基质成分和分子量较小的细胞因子 ,以及结构或功能原始的蛋白分子在猪与人皮肤有较好的同源性 ,而进化较大的蛋白分子 (如细胞因子或激素的受体和淋巴细胞CD分子等 )则多为阴性 ,显示抗体 -抗原结合反应的特异性及其种族差异。结论 :猪与人皮肤组织抗原之间存在很大的差异 ,猪血管内皮细胞表达人ICAM - 1 ,提示二者粘附分子可能存在物种间的交叉 ,可能与异种皮肤移植难以存活有关。本研究为异种脱细胞真皮基质的临床应用提供了有用的资料。Objective:To investigate the difference of antigenicity between human and pig skin..Methods:Frozen section of full thickencss skin of human and pig were stained with immunohistochemical method,using the following antibodies:HLA-ABC,HLA-DR,vimentin,desmin,actin,FⅧRA,cytokeratin,hyaluronic acid,fibronectin,laminine,typeⅠ、Ⅲ、Ⅶ and Ⅶ collagen,estradiol-17 β,and testosterone.Results:The antigens of collagen of types Ⅰ、Ⅲ、Ⅳ、Ⅶ,hyaluronic acid,fibronectin,laminin,FⅧRA,cytokeratin,IL-6,IL-8,estradiol-17 β,testosteron,and ICAM-1 were detected positive only in pig skin.The positive rate was higher with polyclonal antibodies than with in monoclonal antibodies.There are relatively good autoploidy of extracellular matrix(ECM),cytokins with smaller molecules,and proteins with primitive functions,such as IL-6,LI-8,ICAM-1,estradiol-17 β and testosterone in skin of human and pig.However,the autoploidy of recipents of cytokins and CD(cluster of differentiation)molecule in skin were relatively poor.The results showed the specificity and species variation of antigen and antibodies.Conclusions:Antigens are different between human skin and pig skin.This may be the possible reason for rapid rejection following xeno-skin grafting.

关 键 词：免疫组织化学 抗原 人皮肤组织 猪皮肤组织 动物模型 多克隆抗体 

分 类 号：R622.1[医药卫生—整形外科]