静脉应用bFGF对兔缺血心肌血管新生的影响  

Effect of Intravenous Administration of Basic Fibroblast Growth Factor on Ischemic Myocardium in Rabbits

作　　者：张小勇[1] 谭强[1] 李易[1] 光雪峰[1] 马雁冰[2] 陆映珠[1] 

机构地区：[1]昆明医学院附属第二医院心内科,云南昆明650101 [2]中国医学科学院协和医科大学昆明生物研究所

出　　处：《中国微循环》2003年第4期224-226,共3页Journal of Chinese Microcirculation

基　　金：云南省自然科学基金资助项目 (编号 :2000C0109M)

摘　　要：目的探讨静脉给予碱性成纤维细胞生长因子 (bFGF)对兔缺血心肌血管新生的影响。方法开胸结扎兔的冠状动脉左前降支 (LAD) ,制成急性心肌梗塞模型 ,动物被随机分为两组 :一为bFGF组 ,连续2周静脉内给予bFGF(100ug/kg) ;一为对照组 ,连续2周静脉内给予生理盐水2ml。12周时 ,处死动物 ,通过组织学观察两组兔子缺血心肌的血管新生情况。结果bFGF组缺血心肌处的血管密度与对照组比较差异无显著性 (P>0.05)。结论静脉内连续2周给予bFGF(100ug/kg)无明显促进缺血心肌血管新生的作用。Objective To examine the angiogenic effect of intravenous administration of basic fi-broblast growth factor(bFGF)on ischemic myocardium.Methods A rabbit model of myocardial infarction was created by opening chest and ligating the left anterior descending coronary artery(LAD),the animals were randomized to receive bFGF(100ug/kg body weight,n=8)or saline(n=7)by venous injection for14days.12weeks later,vessels growth of ischemic myocardium of each group was evaluated by histological analysis.Results bFGF treatment resulted in no significant increase in capillary density(147.25±24.4/mm 2 vs142.71±23.75/mm 2 ;P>0.05).Conclusions The current investigation shows that intravenous administration of bFGF had no significant angiogenic effect on ischemic myocardium.

关 键 词：碱性成纤维细胞生长因子 心肌缺血 兔 血管新生 

分 类 号：R542.2[医药卫生—心血管疾病]