甲氧基聚乙二醇遮蔽猕猴红细胞表面类人B抗原的研究  

Chemical camouflage of like human B antigens on Rhesus monkey red blood cells with methoxy polyethylene glycol

作　　者：王捷熙[1] 刘泽澎[1] 曹琼[2] 周华勇[2] 兰炯采[2] 章扬培[1] 

机构地区：[1]军事医学科学院野战输血研究所,北京100850 [2]第一军医大学南方医院

出　　处：《中国输血杂志》2003年第4期235-239,共5页Chinese Journal of Blood Transfusion

基　　金：国家 973计划资助课题 (编号 :2 0 0 2CB713 80 4)

摘　　要：目的　观察mPEG对猕猴类人B抗原的修饰效果及mPEG修饰后的红细胞给动物输血的安全性。方法　用 3mg/ml的mPEG修饰遮蔽猕猴红细胞表面的类人B血型抗原 ,吸收放散法检测猕猴血型及mPEG对猕猴红细胞血型的修饰结果 ;红细胞变形仪等检测mPEG修饰对红细胞的变形能力、结构与功能的影响 ;荧光素标记 ,自体回输实验检测红细胞存活率 ;异体回输实验观察修饰后红细胞对受血猴的血液、尿液的影响。结果　mPEG修饰可以遮蔽猕猴类人B抗原的抗原性 ,不影响所修饰红细胞结构、功能及体内存活 ;mPEG修饰的类人B型红细胞输给类人A型血的猕猴 ,未发生输血反应 ;受血猴的血细胞、血液生化及尿液各项指标与输血前相比 ,无明显变化。结论　mPEG修饰可遮蔽猕猴红细胞表面类人B抗原的抗原性 ,mPEG修饰红细胞在受血猴体内是安全的。Objective To observe the chemical camouflage effects of methoxy polyethylene glycol(mPEG) on like human B antigens on Rhesus monkey red blood cell(rRBC) and to study the safety of transfusion with mPEG modified monkey RBC(mPEG rRBC).Methods rRBC were modified with 3mg/ml mPEG. The rRBC blood group and the effects of mPEG modification on blood conversion were assayed by absorption and elution methods.The structural and functional changes of mPEG rRBC were also tested.The survival fraction of mPEG modified like B rRBC in like A recipient monkey were examined by flow cytometry.The transfusion effects of mPEG modified rRBC on recipient's blood and urine were also observed.Results mPEG have been covalently bound to the surface of Rhesus monkey RBC (like human B blood group),mPEG could cover rRBC like B antigen.Transfusion of mPEG modified like B rRBC to like A Rhesus monkey had no harmful effects on the recipient.Conclusion The present study suggests that transfusion of mPEG modified RBC could be safety in experimental animals

关 键 词：甲氧基聚乙二醇 猕猴 化学修饰 类人B抗原 

分 类 号：O633.1[理学—高分子化学] R457.1[理学—化学]