因素重构分析法在硫霉素发酵中的应用  

Application of factor system reconstructability analysis method in the fermentation of thienamycin-producing strain

作　　者：崔大鹏[1] 王丽非[1] 石莲英[1] 

机构地区：[1]中国医学科学院中国协和医科大学医药生物技术研究所,北京100050

出　　处：《中国抗生素杂志》2003年第9期520-523,共4页Chinese Journal of Antibiotics

基　　金：国家九五攻关项目

摘　　要：应用因素重构分析法来改良硫霉素产生菌卡特利链霉菌 PRu- 336 (Streptomyces cattleya PRu-336 )发酵培养基 ,将初步积累的实验数据输入一般化重构分析程序 (Genrecx) ,根据计算的结果 ,设计了 F5 A和 F5 B培养基 ,F5 A效价比对照培养基 F5提高 5 2 .6 % ,F5 B效价比对照培养基 F5提高 36 .8%。Factor system reconstructability analysis method was performed to optimize the medium composites of the thienamycin-producing strain Streptopmyces cattleya PRu-336 in fermentation. The basic data was put into a procedure of factor system reconstructability analysis method. Media F5A and F5B were designed according to the result of calculation. Compared with medium F5, the relative titer of medium F5A was increased by 52.6% and the relative titer of medium F5B was increased by 36.8%.

关 键 词：硫霉素 卡特利链霉菌 因素重构分析法 一般化重构分析程序 

分 类 号：TQ465.1[化学工程—制药化工]