组织工程化骨修复猕猴长段骨缺损的实验研究  

REPAIR OF SEGMENTAL BONE DEFECTS IN RHESUS MONKEYS' RADIUS WITH ALLOGENEIC TISSUE ENGINEERED BONES

作　　者：阳富春[1] 杨志明[1] 李秀群[1] 周悦婷[1] 林凡[1] 秦廷武[1] 罗静聪[1] 解慧琪[1] 

机构地区：[1]四川大学华西医院骨科,成都610041

出　　处：《中国修复重建外科杂志》2003年第5期406-410,共5页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家自然科学基金重点资助项目 (39830 1 0 0 ) ;国家高技术研究发展计划 (863计划 )资助项目 (2 0 0 2 AA2 0 50 1 1 )~~

摘　　要：目的　探讨用生物衍生骨材料和骨髓基质干细胞 (MSCs)复合后构成的组织工程化骨对异体猕猴长段骨缺损的修复作用。　方法　于猕猴胫骨结节抽取 MSCs并使诱导分化为成骨样细胞 ,用 5 -溴脱氧尿嘧啶核苷(Brd U )标记 ,培养后与人源生物衍生骨材料体外复合构成组织工程化骨 ,植入 15只异体猕猴修复桡骨 2 .5 cm长段骨缺损作为实验组 ;用单纯生物衍生骨材料修复对侧同样骨缺损作为对照组 ;另取 2只猕猴双侧桡骨同样部位和大小骨缺损旷置作为空白组。术后 1、2、3、6和 12周时各处死 3只动物取材 ,空白组 12周取材 ,行大体观察、组织学和免疫组织化学检测。　结果　实验组和对照组术后 1、2和 3周移植物周围组织反应较明显 ,6周后明显减轻 ,12周时基本消失。实验组标记成骨样细胞于术后 6周仍存在 ,术后 12周基本消失 ;骨缺损部位骨样组织、软骨、编织骨和板层骨出现时间均较对照组早 ,且骨愈合时间提前 3～ 6周。实验组骨缺损以多点方式直接成骨 ,对照组则从两端以“爬行替代”方式成骨。空白组术后 12周骨缺损均无愈合。　结论　生物衍生骨材料和 MSCs复合构建的组织工程化骨异体植入修复猕猴长段骨缺损可超越骨段移植的“爬行替代”过程 ,使骨缺损能较快愈合。生物衍生骨材料和同种异体 MSCs复合组织工程?Objective To construct tissue engineering bone with bio derived materials and bone marrow stromal cells (MSCs), and to investigate the effect of allogeneic engineering bone implants on healing of segmental bone defects. Methods MSCs being aspirated aseptically from tibial tuberosities of young rhesus monkeys were induced into osteoblasts in vitro and then were cultured and marked with 5 bromo 2 deoxyuridine (BrdU).Tissue engineering bones were constructed with these labeled osteoblasts being seeded onto bio derived materials made from fresh human bones which were treated physically and chemically, Then the constructs were implanted in 15 allogeneic monkeys to bridge 2 5 cm segmental bone defects of left radius as experimental groups, bio derived materials only were implanted to bridge same size defects of right radius as control group. and, 2.5 cm segmental bone defects of both sides of radius were left empty in two rhesus monkeys as blank group. Every 3 monkeys were sacrificed in the 1st, 2nd, 3rd, 6th and 12th weeks postoperatively and both sides of the implants samples were examined macroscopically, histologicaly, and immunohistochemicaly. The two monkeys in blank group were sacrificed in the 12th week postoperatively. Results Apparent inflammatory reactions were seen around both sides of the implants samples in the 1st, 2nd , 3rd weeks, but it weakened in the 6th week and disappeared at the 12th week. The labeled osteoblasts existed at the 6th week but disappeared at the 12th week. The bone defects in experimental group were repaired and the new bone formed in multipoint way, and osteoid tissue, cartilage, woven bone and lamellar bone occurred earlier when compared with control group in which the bone defects were repaired in 'creep substitution' way. The bone defects in blank group remained same size at the 12th week. Conclusions Engineering bones constructed with bio derived materials and MSCs were capable of repairing segmental bone defects in allogeneic monkeys beyond 'creep substitution' way

关 键 词：组织工程化骨 长段骨缺损 修复 猕猴 实验研究 异体移植 生物衍生材料 手术方法 

分 类 号：R318.17[医药卫生—生物医学工程]