中药调控血小板衍化生长因子逆转早期糖尿病肾病机制的实验研究  

Experimental Study on the Mechanism of Drug Changing Early Diabetic Nephropathy by Regulating Platelet-Derived Growth Factor-β

作　　者：唐红[1] 徐蓉娟[1] 李红[1] 葛芳芳[1] 胡健炜[1] 

机构地区：[1]上海中医药大学附属龙华医院,上海200032

出　　处：《中国中西医结合肾病杂志》2003年第9期510-513,共4页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：上海市卫生局中医药科研资助项目 (No .2 0 0 3G0 1)

摘　　要：目的 :观察益气活血补肾的治糖保肾冲剂对早期糖尿病肾病 (DN)大鼠肾皮质血小板衍化生长因子 -β(PDGF - β)蛋白及PDGF - βmRNA表达的影响 ,探讨其作用机理。 方法 :实验采用链脲佐菌素 (STZ)及福氏佐剂(CFA)诱发糖尿病肾病大鼠模型 ,造模成功后随机分为正常组、模型组、中药组 (治糖保肾冲剂 )、西药组 (苯那普利 ) ,治疗 4周后检测大鼠肾皮质PDGF - β蛋白及PDGF - βmRNA表达。 结果 :中药组治疗后尿微量白蛋白 (Ualb)明显低于模型组 ,有统计学差异 (P <0 .0 5 ) ;中药组治疗后PDGF - β蛋白及PDGF - βmRNA表达均明显低于模型组 (P <0 .0 1,P <0 .0 5 )。结论 :提示治糖保肾冲剂治疗早期DN的机理与抑制PDGF - β蛋白及PDGF -Objective:To study the mechanism of “ZhiTangBaoShen”powder preparation with supplementing Qi, activating blood circulation and tonifying kidneys by detecting the espression of PDGF-β protein and mRNA in renal cortex in rat diabetic nephropathy model.Methods:The rat DN model was established by injection of STZ. The rats were randomly divided into normal group, model group, group treated with “ZhiTangBaoShen”powder and group treated with ACE inhibitor. The expression of PDGF-β protein and of PDGF-β mRNA were detected after 4 weeks. Results:There was obvious difference between model group and group treated with “ZhiTangBaoShen”powder in urine albumin ( P <0.05), the expression of PDGF-β protein and of PDGF-β mRNA in renal cortex( P <0.01, P <0.05 respectively).Conclusion:The mechanism of ZhiTangBaoShen powder in treating early diabetic nephropathy might be closely linked with its suppression of the expression of PDGF-β protein and of PDGF-β mRNA in renal cortex.

关 键 词：中药 血小板衍化生长因子 早期 糖尿病肾病 实验 治糖保肾冲剂 

分 类 号：R277.51[医药卫生—中医学]