肝移植术后致敏状态的监测  

Detection of sensitization to HLA antigens after liver transplantation

作　　者：张伟杰[1] 王大卫[1] 程敦秀[1] 叶启发[1] 陈知水[1] 曾凡军[1] 李岗山[1] 宫念樵[1] 陈忠华 

机构地区：[1]华中科技大学同济医学院附属同济医院器官移植研究所,武汉430030

出　　处：《中华器官移植杂志》2003年第5期267-268,共2页Chinese Journal of Organ Transplantation

摘　　要：目的　探讨肝移植受者致敏与移植物功能的关系。方法　采用酶联免疫吸附法(ELISA)对 15例患者肝移植前后的群体反应性抗体 (PRA)进行动态监测。结果　 15例患者肝功能的主要指标 ,包括丙氨酸转氨酶、总胆红素、直接胆红素等大多在术后 1周内恢复正常。 15例中有 1例术前中度致敏 ,术后发生轻度排斥反应 1次 ,经治疗后逆转 ,其PRA在观察期内一直保持阳性。另外 14例受者移植前、后PRA均阴性 (PRA <10 % ) ,其中 1例发生排斥反应 1次 ,但PRA仍保持阴性。结论　肝移植受者的致敏状态与术后的排斥反应密切相关。Objective To investigate the sensitization to HLA antigens and relationship between sensitization and allograft function after liver transplantation. Methods The panel reactive antibody (PRA) in serum was determined by using LAT ELISA kit in 15 recipients before and after liver transplantation. Results Moderate sensitization was detected in one of 15 cases before transplantation. The patient experienced one episode of mild rejection, which was reversed by treatment with Methylprednisolone. PRA in this patient maintained positive 3 months posttransplantation. The remaining 14 patients had negative PRA (<10 %) before and after surgery. One recipient developed acute rejection, but the PRA was continually negative. Conclusion Sensitization rate in Chinese liver recipients is lower, which might be related to the lower incidence of rejection after liver transplantation.

关 键 词：肝移植术 术后 致敏状态 酶联免疫吸附法 群体反应性抗体 

分 类 号：R657.3[医药卫生—外科学]