半透膜包裹胰岛脑内移植延长异种胰岛的生存期  

Prolongation of survival peirod of islets by ICCs encapsulated by semipermeable membrane xenografted in brain

作　　者：信照亮[1] 胡海涛[2] 贾延杰[2] 任惠民[2] 

机构地区：[1]西安交通大学第二医院神经外科 [2]西安交通大学医学院解剖教研室

出　　处：《中华器官移植杂志》2003年第5期313-315,共3页Chinese Journal of Organ Transplantation

基　　金：陕西省自然科学基金资助项目 ( 98SZ 0 6 4)

摘　　要：目的　研究生物半透膜包裹胰岛样细胞团 (ICCs)的免疫隔离效果。方法　分离培养SD大鼠的ICCs,用生物半透膜包裹 ,体外观察半透膜的通透性 ,并将包囊后的ICCs植入犬大脑实质内 ,术后 1个月和 2个月以光镜和电镜观察移植物和周围脑组织的病理改变 ,免疫组化染色鉴定移植物内的胰岛B细胞。结果　半透膜包囊在整个体外实验过程中无破损 ,淋巴细胞及其它血细胞成份不能透过半透膜 ,而葡萄糖和胰岛素能迅速弥散透过半透膜囊。移植后 2个月 ,移植物内无多形核细胞和淋巴细胞的团状浸润 ,免疫组化染色证明移植物内有胰岛B细胞存在 ,周围的脑组织仅有轻度的胶质细胞增生和水肿。结论　半透膜包裹ICCs结合免疫特赦部位移植的方法对克服异种胰岛移植的免疫排斥反应有一定的作用。Objective To identify the effect of decreasing the rejection using the biological semipermeable membrane combined with the privileged site xenotransplantation. Methods The rat ICCs encapsulated by biological semipermeable membrane were xenotransplanted into dog's brain. The pathological changes of implants and surrounding cerebral tissues were observed under light and electric microscopy. The β-cells of implants were identified by immunohistochemistry. Results After 2 months of transplantation, The β-cells were observed and the lymphocytes were dispersed in the grafts.The brain tissues near the grafts showed slight edema and glial hyperplasia. Conclusion The method using the biological semipermeable membrane in combination with the privileged site xenotransplantation have a beneficial effect on the inhibition of the rejection of heterogeneous ICCs implant.

关 键 词：异种胰岛移植 生物半透膜 免疫隔离效果 排斥反应 

分 类 号：R651.1[医药卫生—外科学]