中药M对兔内皮细胞增殖影响的初步研究  

Preliminary Study about the Active Ingredients of Chinese Herb M Influencing Proliferation of Endothelial Cells in Rabbit

作　　者：樊粤光[1] 梁笃[1] 刘建仁[1] 李雄[1] 王海彬[1] 何伟[1] 张德兴[1] 范光顺[1] 杨荣建[1] 

机构地区：[1]广州中医药大学,广州510405

出　　处：《中国实验动物学报》2003年第3期181-183,共3页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金科研项目 (基金号 :3 0 17114 3 );广东省自然科学基金科研项目 (基金号 :0 10 2 5 1)

摘　　要：目的　旨在探讨中药M对兔内皮细胞增殖的影响 ,试图阐明单味中草药品种M的活性成分促进血管生成的生物学机制。方法　建立兔胸主动脉内皮细胞体外培养模型 ,制备不同浓度的兔中药血清加入 96孔内皮细胞培养板 ,以MTT比色法测定内皮细胞增殖情况。结果　生脉素能够促进内皮细胞的增殖 (P <0 0 5 )。Objective To study the Chinese herb M on influencing proliferation of endothelial cells and to elucidate its biological mechanism of vascularization. Methods The model of rabbit chest aorta endothelial cells was established in vitro, and rabbit sera with different concentration of the drug were prepared. The serum was added into 96 wells with endothelial cells in culture plate, and the proliferation of endothelial cells was measured with MTT colorimetry. Result the Chinese herb M can promote proliferation of endothelial cells.Conclusion The Chinese herb M may improve vascularization by promoting proliferation of endothelial cells.

关 键 词：兔 内皮细胞 增殖 中草药 活性成分 

分 类 号：R285.5[医药卫生—中药学]