菌多糖对Balb/c 3T_3、Hela、HEK-293细胞增殖的影响  

The Effect of Polysaccharides from Microbe on the Proliferation of Balb/c 3T_3 , Hela and HEK-293 Cells

作　　者：刘士德[1] 丘劲[1] 张建华[1] 邢苗[1] 

机构地区：[1]深圳大学生命科学学院,深圳518060

出　　处：《深圳大学学报（理工版）》2003年第3期58-63,共6页Journal of Shenzhen University(Science and Engineering)

基　　金：深圳市科技局资助项目(200108);深圳大学科研基金(199802)

摘　　要：用苯酚法提取JM109大肠杆菌、O157大肠杆菌、霍乱弧菌、伤寒杆菌及多头绒泡菌的菌多糖,用MTT法检测菌多糖在体外对Balb/c 3T_3(鼠成纤维细胞)、Hela(人上皮细胞)和HEK-293(人胚肾细胞)增殖的影响.结果表明,菌多糖在低质量浓度(低于40mg/L)时抑制Hela细胞和HEK-293细胞的增殖,但促进Balb/c 3T_3细胞的增殖;菌多糖超过一定质量浓度(80mg/L)时,对Balb/c 3T_3细胞的增殖作用和对Hela、HEK-293细胞增殖的抑制作用趋于饱和.The effect of polysaccharides on the proliferation of Balb/c 3T3, Hela and HEK-293 cells was determined by MTT. The polysaccharides were extracted from JM109 E. coli , 0157 E. c-oli, vibrio cholerae, typhoidin and Physarum polycephalum by phenol. The results showed that low concentration polysaccharides inhibit the proliferation of Hela and HEK-293 cells, but facilitate the proliferation of Balb/c 3T3 cells. The effect on the proliferation of Balb/c 3T3, Hela and HEK-293 cells reaches a constant value for polysaccharides with their concentration exceeding 80 mg/L.

关 键 词：菌多糖 MTF 细胞增殖 移植性肿瘤 鼠成纤维细胞 Balb/c3T3 HELA 人上皮细胞 HEK-293 人胚肾细胞 

分 类 号：Q253[生物学—细胞生物学]