牛布氏杆菌病热酚法脂多糖抗原酶联免疫吸附试验(ELISA)的研究  

Studies on ELISA for Detection of Bovine Brucellosis with Heating - phenol Extracted LPS Antigen

作　　者：张喜悦[1] 王志亮[1] 王君玮[1] 吴延功[1] 徐天刚[1] 徐培莲[1] 赵云玲[1] 

机构地区：[1]农业部动物检疫所,青岛266032

出　　处：《中国动物检疫》2003年第10期18-20,共3页China Animal Health Inspection

摘　　要：采用世界动物卫生组织(OIE)推荐的热酚法提取的脂多糖(LPS)作为抗原,建立了牛布氏杆菌病的ELISA方法,对45份血清进行检测,证明本ELISA方法灵敏性较高,经阻断试验和交叉试验,证明特异性较好。同时对建立的ELISA方法进行了改进,使制备的牛布氏杆菌ELISA试验试剂盒能在4℃条件下长期保存,具有良好的稳定性和重复性。An ELISA(enzyme-linked immunosorbent assay.) was developed for detection of bovine brucellosis by using LPS (lipopolysaccharide) as antigen purified by Heating - phenol recommended by OIE (office international des epizootics) . It indicated the ELBA is sensitive by detecting 45 cattle and specific by blocking test and cross - reacting. Bovine brucellosis ELISA kit was developed based on the study. It was stable at 4℃ for long term, with good repetition.

关 键 词：牛 布氏杆菌病 热酚法 脂多糖抗原 酶联免疫 吸附试验 抗原制备 

分 类 号：S858.23[农业科学—临床兽医学]