益生芽孢杆菌B.licheniformis29质粒pBL29性质的研究  

Studies on plasmid pBL29 of B.licheniformis 29

作　　者：刘一尘[1] 倪学勤[2] 王淑芳[1] 何明清[2] 李宏伟[1] 

机构地区：[1]河南科技大学动物科技学院,河南洛阳471003 [2]四川农业大学动物科技学院,四川雅安625014

出　　处：《河南科技大学学报（农学版）》2003年第2期52-57,共6页

摘　　要：质粒pBL29为闭合环状质粒,酶切分析证明质粒pBL29有大量的单酶切位点,如EcoRⅠ、SmaⅠ、HindⅢ、BglⅡ、PstⅠ、XbaⅠ、BamHⅠ等,并根据限制性酶切各片段的分子量作出了质粒pBL29的内切酶图谱。对质粒pBL29进行测序和分析,证明了其大小为3711bp,具有大量的单酶切位点、编码卡那霉素抗性基因以及富含A/T序列的复制起点。The plasmid pBL29 is cccDNA and the molecular weight of the plasmid pBL29 was estimated to be about 3.7kb. The character of the plasmid is its many unique restriction endonucleases sites, such as EcoR　Ⅰ, Sma　Ⅰ, Hind　Ⅲ, Bgl　Ⅱ, Pst　Ⅰ, Xba　Ⅰand BamH Ⅰ et al. The restriction map of the plasmid pBL29 was tentatively constructed according to the molecular weights of fragments of plasmid pBL29 digested with different enzymes. Analyzing the sequence of the plasmid pBL29 tested, we found that the molecular weight of the plasmid pBL29 is 3711bp,that it has many unique restriction endonucleases, Km resistance genes and abundant A/T base pairs of replicating site are on the plasmid pBL29.

关 键 词：益生芽孢杆菌 B.licheniformis29质粒 pBL29性质 抗性因子 酶标记 卡那霉素 

分 类 号：Q939.11[生物学—微生物学]