严重急性呼吸综合征(SARS)冠状病毒刺突蛋白基因片段的表达及其初步应用  

Cloning and Expression of SARS Cor onavirus Spike Gene Fragment and It s Application

作　　者：伊瑶[1] 段淑敏[1] 张明程[1] 周永东[1] 高阳[2] 杨焕明[3] 董小平[1] 毕胜利[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京100052 [2]广州市疾病预防控制中心,广州510080 [3]中国科学院华大基因研究中心,北京100101

出　　处：《病毒学报》2003年第3期267-268,共2页Chinese Journal of Virology

基　　金：863计划重大项目"非典型肺炎防治关键技术及产品研制"资助 (2 0 0 3AA2 0 82 0 4)。

摘　　要：SARS virus spike gene fra gment was expressed by Ecoli expr ession systemThe fragment enclose s major neutralization epitope of the virusThe expressed protein wa s purified and an ELISA method was set upBy using the recombinant,tw elve patients’ sera were detected The recombinant SARS coronavirus s pike protein offers an efficient wa y for serological diagnosis and is useful for epidemiological survey and vaccin eSARS virus spike gene fra gment was expressed by Ecoli expr ession systemThe fragment enclose s major neutralization epitope of the virusThe expressed protein wa s purified and an ELISA method was set upBy using the recombinant,tw elve patients' sera were detected The recombinant SARS coronavirus s pike protein offers an efficient wa y for serological diagnosis and is useful for epidemiological survey and vaccin e development

关 键 词：严重急性呼吸综合征 SARS 冠状病毒 刺突蛋白 基因片段 表达 

分 类 号：R373.1[医药卫生—病原生物学] R563.1[医药卫生—基础医学]