猪伪狂犬病毒血清学调查及部分特性的研究  

Serology Investigation of Pseudorables Virus and Study on Some Characters

作　　者：宋春阳[1] 单虎[1] 韩先杰[1] 宋玉芹[1] 齐香荣[1] 杨斌成 

机构地区：[1]莱阳农学院动物科技学院,山东莱阳265200 [2]沂源县畜牧局

出　　处：《莱阳农学院学报》2003年第3期217-219,共3页Journal of Laiyang Agricultural College

摘　　要：从山东某猪场疑似猪伪狂犬病死亡的仔猪大脑、扁桃体等组织中分离到一株病毒。该病毒接种家兔后,使家兔产生典型的奇痒症状,且能在鸡胚成纤维细胞(CEF)上增殖,并可产生典型的细胞病变(CPE)。CEF细胞接种该病毒后,进行DNA琼脂糖凝胶电泳时,呈现"梯状"条带,说明病毒感染CEF细胞后,使细胞产生"凋亡"现象。综合本病毒的各种特性,初步鉴定为伪狂犬病毒。应用伪狂犬乳胶凝集诊断试剂盒对来自山东6个猪场共100份血清进行了血清学调查。共检出阳性血清25份,血清阳性率达25%。One strain virus was isolated from a pig farm in ShanDong province. Chicken embryo fiber cell (CEF) could multiply the virus and was caused typical Cytopathic effect(CPE). Also,the virus could make CEF appear the phenomenon of 'Apoptosis'. Rabbit which was inoculated the virus itched seriously. The experiments showed the virus isolated was Pseudorabies Virus(PRV). The result of serology investigation showed the positive rate was 25%.

关 键 词：猪 伪狂犬病毒 血清学调查 细胞凋亡 

分 类 号：S858.28[农业科学—临床兽医学] S852.65[农业科学—兽医学]