Analysis of parental strain DNA fragments existing in GEMs-Fhhh  

作　　者：HAOChun-bo YANJun QUMeng-meng WANGDong CHENGShu-pei GUJi-dong QIUWan-fei WANGYin-yin 

机构地区：[1]TheNationalKeyLaboratoryofPollutionControlandResourceReuse,SchoolofEnvironmentalScience,NanjingUniversity,Nanjing210093,China [2]DepartmentofEcology&Biodiversity,TheUniversityofHongKong,PokfulamRoad,HongKong,China

出　　处：《Journal of Environmental Sciences》2003年第5期590-594,共5页环境科学学报（英文版）

基　　金：TheNational863HiTechFoundationofChina(No.2 0 01AA214191)andNSFofJiangsuProvince(No.BK 990 33)andtheChinaEnvironmentalProtectionCompanyofHongKong

摘　　要：There were 6 target DNA fragments of the three parental strains existing in the cell of GEMs(genetically engineered microorganism strain) Fhhh measured in this research by PCR(polymerase chain reaction). The determination showed that GEMs Fhhh contained all the 6 target DNA fragments, mnp 1, mnp 2、 lip 1、 lip 2, FLO 1 and 16S rDNA, and had the molecular genetic stability. Meanwhile the PCR production of each parental strain could only had its target DNA fragments and was different from each other. It may illustrate that the technique of the inter kingdom protoplast fusion for the construction of GEMs Fhhh through the process of intercellular gene recombination could be used as a reliable bioengineering technique to create the specific functional stain for the pollution control.There were 6 target DNA fragments of the three parental strains existing in the cell of GEMs(genetically engineered microorganism strain) Fhhh measured in this research by PCR(polymerase chain reaction). The determination showed that GEMs Fhhh contained all the 6 target DNA fragments, mnp 1, mnp 2、 lip 1、 lip 2, FLO 1 and 16S rDNA, and had the molecular genetic stability. Meanwhile the PCR production of each parental strain could only had its target DNA fragments and was different from each other. It may illustrate that the technique of the inter kingdom protoplast fusion for the construction of GEMs Fhhh through the process of intercellular gene recombination could be used as a reliable bioengineering technique to create the specific functional stain for the pollution control.

关 键 词：GEMS protoplast fusion target gene DNA fragment PCR pollution control 

分 类 号：X703[环境科学与工程—环境工程]