海洋适冷菌产低温纤维素酶的条件优化  

Optimization of Fermentation Conditions for Cold-Active Cellulase Produced by Psychrotrophic Bacterium

作　　者：王玢[1] 李志香[1] 袁方曜[2] 

机构地区：[1]山东教育学院生物系,山东济南250013 [2]山东省农科院,山东济南250100

出　　处：《山东教育学院学报》2003年第4期63-64,68,共3页Journal of Shandong Education Institute

摘　　要：本文对一株分离自黄海深海海底的产纤维素酶海洋适冷菌进行了产酶条件研究。结果显示。在接近自然海水盐度、温度20℃,自然pH条件下,菌体生长和产酶较好。所产纤维素酶为诱导酶,主要作用底物为CMC Na和微晶纤维素。以1%球磨Avicel+1%蔗糖构成的复合碳源产酶活力最高;最适氮源为1%蛋白胨+1%酵母膏。One strain of coldadapive cellulaseproducing bacterium which can degrade carboxymethyl Cellulase (CMC) was isolated from the submarine of the Yellow Sea. The fermentation conditions were investigated. The strain grows and produce cellulase well under the condition of the salinity near that of natural seawater, temperature(20℃), and pH. The cellulase showed strong activity toward CMC and Avicel. The efficiency carbon sources were 1% Avices+1% saccharose for the higher production of enzyme, while 1% peptone+1% yeast extract were the best nitrogen sources.

关 键 词：海洋微生物 适冷菌 低温纤维素酶 

分 类 号：Q178.53[生物学—水生生物学]