太平洋牡蛎(Crassostrea gigas)不同组织DNA相对含量及细胞周期的分析  

Analysis of DNA Relative Contents and Cell Cycle of Different Tissues of Crassostrea gigas

作　　者：丁君[1] 常亚青[1] 王子臣[1] 宋坚[1] 胡秀媛 王延斌 

机构地区：[1]大连水产学院农业部海洋水产增养殖学与生物技术重点开放实验室,辽宁大连116023 [2]大连市水产研究所,辽宁大连116013

出　　处：《海洋科学进展》2003年第2期203-208,共6页Advances in Marine Science

基　　金：国家高新技术研究发展计划资助项目--虾夷马粪海胆性别决定机制及单性苗种培育技术的研究(2002AA628170)

摘　　要：以太平洋牡蛎(Crassostreagigas)为试验材料,应用流式细胞仪分别测定常温下(16℃)和0℃低温下鳃、外套膜、性腺(包括2个取样部位)的DNA相对含量并分析各组织细胞周期各时相的差异。试验结果表明,常温下(16℃)牡蛎不同组织DNA相对含量的顺序依次为性腺取样部位2>外套膜>鳃>性腺取样部位1,性腺取样部位1的DNA含量低于前三者(P<0.05);在0℃低温处理后,牡蛎各组织的DNA含量有所下降,随着低温处理时间的延长,鳃组织的DNA含量变化不显著(P>0.05),外套膜的DNA含量显著减少(P<0.01),性腺组织的显著增加(P<0.01);常温下牡蛎各组织细胞停留在G1期时相的比率依次为:性腺取样部位2>鳃>外套膜>性腺取样部位1;在0℃低温处理下,鳃与外套膜之间停留在G1期比率差异不显著(P>0.05),其余各组织间G1期比率差异均极显著(P<0.01),对于同一部位,随着0℃持续时间不同,鳃、性腺取样部位2处于G1期时相比率的变化不显著(P>0.05),外套膜和性腺部位1的变化显著;与常温下相比,0℃处理性腺取样部位1,2后,二者停留在G1期时相的比率均显著增加(P<0.01)。Flow cytometry was used to determine the DNA relative content and analyze the differences between different cell cycle time phases of different tissues of Grassostrea gigas at normal temperature (16 ℃) and low temperature (0 ℃). It is shown from the analysis results that the DNA relative contents of different tissues arrange in order of magnitude to be gonad 2, mantle, gill and gonad 1. The DNA relative contents decreased after Crassostrea gigas being kept at 0 ℃, the change in the DNA relative content of gill was not significant, the DNA relative content of mantle decreased significantly, and that of gonad increased significantly. The ratios for different tissue cells to remain in G_1 phase at 16 ℃ arrange in order of magnitude to be gonad 2, gill, mantle and gonad 1. The difference between the ratios for gill and mantle to remain in G_1 phase at 0 ℃ was not significant, and the differences between the ratios for other tissues to remain in G_1 phase were significant. The changes in ratios for gill and gonad 2 to remain in G_1 phase at 0 ℃ were not significant, and those for other tissues were significant. The ratios for gonad 1 and gonad 2 to remain in G_1 phase at 0 ℃ were significantly greater than those at 16 ℃.

关 键 词：太平洋 牡蛎 DNA相对含量 细胞周期 流式细胞仪 FCM 

分 类 号：Q176[生物学—水生生物学] Q959.215.3[生物学—普通生物学]