年龄对成年小鼠嗅球细胞增殖与神经发生的影响  

Effects of age on cell proliferation and neurogenesis in the adult mouse olfactory bulb

作　　者：张立平[1] 胡坚莉[1] 吴琦[1] 

机构地区：[1]山东大学医学院解剖学教研室

出　　处：《山东大学学报（医学版）》2003年第4期365-367,共3页Journal of Shandong University:Health Sciences

摘　　要：目的:观察年龄对成年小鼠嗅球细胞增殖与神经发生的影响。方法:用5-溴-2-脱氧尿核苷(5-bromo-2’-deoxyuridine,BrdU)标记新生细胞,免疫组化染色后统计新生细胞数量;应用BrdU与TuJ1/GFAP双标记,鉴别出BrdU阳性细胞的细胞类型。结果:老年组小鼠嗅球细胞增殖与神经发生明显低于青年组。结论:成年小鼠嗅球的细胞增殖与神经发生随着年龄的增长而减慢,这可能是嗅觉减退的结构基础之一。Objective:To study the effects of age on cell proliferation and neurogenesis in the adult mouse olfactory bulb.Methods:Proliferating cells were labeled with5-bromo-2'-deoxyuridine(BrdU)for10days.BrdU immunohistochemistry was conducted to dertermine the number of newly generated cells and double immunolabeling undertaken to co-localize BrdU-positive cells with TuJ1(Tubulin-βAb-3,neu-ron-specific early differentiation marker)/GFAP(rabbit anti-glial fibrillary acid protein).Results:The number of new-born cells in the aged mice was significantly lower than that in the young mice.Conclu-sion:Cell proliferation and neurogenesis in the olfactory bulb decrease with the age increase of the adult mice,which may be related to the decrease of odor discrimination in the aged.

关 键 词：溴脱氧尿苷 嗅球 细胞增殖 神经发生 年龄组 小鼠 

分 类 号：Q429.1[生物学—神经生物学] R338[生物学—生理学]