9-顺-维甲酸对人肺鳞癌细胞周期调控因子CyclinD1、Cdk4转录的影响  

Effects of9-cis-retinoic acid on CyclinD1and Cdk4transcription in lung cancer cell line

作　　者：刘志方[1] 任桂杰[1] 丁磊[1] 胡国强[2] 胡晓燕[1] 于雪艳[1] 

机构地区：[1]山东大学医学院生化与分子生物学研究所 [2]山东大学齐鲁医院胸外科

出　　处：《山东大学学报（医学版）》2003年第4期368-370,374,共4页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助课题(39770730)

摘　　要：目的:检测9-顺-维甲酸(9-cis-RA)处理前后人肺鳞癌细胞株L78、A2的细胞周期调控因子Cy-clinD1、Cdk4转录水平的变化。探讨其抑制肺鳞癌细胞生长的作用机制。方法:采用半定量逆转录聚合酶链式反应(RT-PCR)分析细胞周期调控因子CyclinD1、Cdk4转录水平的变化。结果:9-cis-RA对两细胞株的细胞周期调控因子CyclinD1、Cdk4转录水平均有所降低,且具有统计学意义(P<0.05)。结论:9-cis-RA抑制肺鳞癌细胞株L78、A2增殖的作用机理与其调节细胞周期调控因子CyclinD1、Cdk4的转录有关。Objective:To study the changes of CyclinD1and Cdk4mRNA level in human lung cancer cell line L78and A2before and after treatment with9-cis-RA(1μmol/L),and to explore the ef-fecting mechanism of9-cis-RA on the proliferation of lung cancer cells.Methods:The mRNA expressions of CyclinD1and Cdk4of human lung cell line L78and A2were determined by RT-PCR.Results:9-cis-RA significantly reduced the mRNA expressions of Cdk4and CyclinD1in lung cancer cell line L78and A2.Conclusion:The growth inhibitory effect of9-cis-RA on lung cancer cells is tightly correlated with its declining the mRNA expression of Cdk4and CyclinD1.

关 键 词：维甲酸 肺肿瘤 聚合酶链式反应 转录 细胞周期蛋白D1 Cdk蛋白质类 

分 类 号：R977.21[医药卫生—药品] R734.2[医药卫生—药学]