碱性成纤维细胞生长因子对大鼠真皮下血管网皮片愈合的影响  

Influence of basic fibroblast growth factor on the healing of subcutaneous vascular net free skin graft in Wistar rats

作　　者：赵焕童[1] 李湘洲[2] 王歧刚[1] 刘海鹰[1] 周晓力[3] 邓诗琳[4] 

机构地区：[1]潍坊市人民医院烧伤整形,山东省潍坊市261041 [2]潍坊市人民医院病理科,山东省潍坊市261041 [3]潍坊市人民医院烧伤整形科,山东省潍坊市261041 [4]天津市烧伤研究所,天津市300222

出　　处：《中国临床康复》2003年第23期3168-3169,T001,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:探讨碱性成纤维细胞因子(bFGF)对大鼠真皮下血管网皮片愈合的影响。方法:Wistar大白鼠30只,每只背部切取2个真皮下血管网皮片并原位移植缝合,上部皮片下注入bFGF为实验组。下部皮片为对照组,术后大体肉眼观察皮片成后情况,并于2,4,6,8d取材镜下组织学观察CD34阳性细胞数、血管内皮细胞生长情况,微血管密度及肉芽组织生长情况。结果:实验组与对照组皮片成活率分别为93%及64%,经配对t检验差异显著(t=3.701,P<0.01)。其镜下观察CD34阳性细胞数、微血管数及肉芽组织厚度两组均有明显差异(P<0.01)。结论:bFGF具有广泛的细胞增殖效应,可通过多个环节促进小血管形成及肉芽组织增长,加速移植皮片与周围皮肤的愈合过程。AIM: To explore the influence of basic fibroblast growth factor (bFGF) on the healing of subcutaneous vascular net free skin graft in rats. METHODS:Two skin grafts of subcutaneous vascular net were cut off in the back of 30 Wistar rats respectively and then transplanted in situ. The upside skin grafts used as test group, were injected with bFGF, and downside skin grafts were used as control group. After operation, the survival of skin grafts was generally observed by naked eye.Histologically, the skin grafts were sampled to observe CD34 positive cell number, survive situation of vascular endothelial cells, microvessel density(MVD) and growth of granulation tissues with microscope. RESULTS:The survival rates of skin grafts in the test and control groups were 93% and 64% respectively (t=3.701,P< 0.01).Under the microscope, there were significant differences in the number of CD34 positive cells, MVC and depth of granulation tissues between the two groups (P< 0.01).

关 键 词：碱性成纤维细胞生长因子 大鼠 真皮下血管网皮片愈合 影响 外科皮瓣 

分 类 号：R622.1[医药卫生—整形外科]