人胚胎嗅鞘细胞植入鼠半横断脊髓的初步观察(英文)  

Preliminary observation of the effect of transplantation of human embryonic OECs on adult SD rats' semitransected spinal cord

作　　者：王学文[1] 唐勇[1] 吴燕峰[2] 殷德振[1] 杨睿[1] 黄霖[1] 沈慧勇[1] 

机构地区：[1]中山医科大学孙逸仙纪念医院骨外科,广东省广州市510120 [2]中山医科大学岭南脐血库,广东省广州市510120

出　　处：《中国临床康复》2003年第23期3174-3175,T003,共3页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金(30271311);广东省科技厅攻关项目(C30702)~~

摘　　要：目的观察人胚胎嗅鞘细胞(olfactoryensheathingcells,OECs)移植对成鼠脊髓轴突再生的影响。方法将分离、纯化、培养2周的人胚胎OECs,移植于10只成年SD大鼠半横断胸髓(T10)的两端;对照组10只同样方法给予等量的DMEM培养液。6周后组织切片,行HE染色、嗜银染色以及髓鞘碱性蛋白(MBP)和抗神经生长因子受体抗体(NGFR)免疫组化检查。结果OECs移植组6周后脊髓大体标本显示初步愈合现象;组织学观察,OECs呈NGFR阳性表达,显示OECs仍然存活,且与宿主组织整和良好;部分再生轴突长入断端间组织,呈MBP阳性表达。结论人胚胎OECs对成鼠胸髓损伤后的轴突再生有促进作用。AIM:To observe the effect of transplantation of human embryonic OECs on adult rats' semitransected spinal cord. METHODS: OECs,separated from human embryonic olfactory bulb and purified by culture in vivo for 2 weeks,were transplanted into SD rats'(n=10) semitransected spinal cord at T10 level,while the vehicle solution (DMEM) was injected in the controls(n=10).Samples were procured at 6 week after transplantation and studied by nerve silver staining and myelin basic protein(MBP) histochemical staining and nerve growth factor receptor(NGFR) immunohistochemical staining.RESULTS:The injured spinal cords displayed initial healing in OECs transplanted rats 6 weeks post transplantation morphologically.Histological observation showed that the transplanted OECs integrated into the host tissues in well arrangement and the regenerative axons grew into the distal end of the semitransected spinal cords.Both of OECs and regenerative axons positively expressed MBP and NGFR.In control group, the ends of the impaired spinal cords exhibited liquefied necrosis and axonal degeneration and no regnnerative axons were found.

关 键 词：人胚胎嗅鞘细胞植入 鼠 半横断脊髓 观察 组织切片 嗜银染色 髓鞘碱性蛋白 免疫组化 脊髓轴突再生 神经再生 

分 类 号：R651.2[医药卫生—外科学]