不同应力对人牙周膜成纤维细胞细胞骨架影响的体外研究  

A study on the effects of different stress on cytoskeleton of human periodontal fibroblasts in vitro

作　　者：杨美祥[1] 林珠[1] 丁寅[1] 

机构地区：[1]解放军第四军医大学口腔医学院正畸科,陕西省西安市710032

出　　处：《中国临床康复》2003年第23期3162-3163,T002,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:研究不同应力对体外培养的人牙周膜成纤维细胞微丝形态的影响。方法:分别给第3～5代的人牙周膜成纤维细胞施加静压力、周期性牵张力和流体剪切力,用相差显微镜和考马斯亮蓝染色方法观察加力后不同时间细胞及其微丝形态的变化特征。结果:流体剪切力作用6h后,部分人牙周膜成纤维细胞出现收缩、微丝排列等方面的变化;12h后,部分细胞死亡、脱落。较大的静压力(>100kPa)作用12h后,可引起细胞间隙增大等变化,细胞骨架改变较少。结论:一定性质和力量的外力可引起体外培养的人牙周膜成纤维细胞微丝的重排、细胞贴壁及细胞形态的变化。静压力与剪切力、动态张力相比,对体外培养的人牙周膜成纤维细胞微丝形态影响较轻。AIM:To study the effects of different stress on microfilaments of human periodontal fibroblasts cultured in vitro.METHODS:Cyclic strain force, fluid shear stress and compressive pressure force were applied on the third fifth passage human periodontal ligament cells respectively. Morphological changes of cells and filaments were observed in different intervals using phase contrast microscope and Coomassie BB staining.RESULTS:The rearrangement of filaments and cell shrinkage occurred at 6 h after cyclic fluid shear stress, and some cells died and fell off after 12 h. Whereas there were no markedly changes after12 h compressive pressure (>100 kPa) except slight cell contract and increased space between cells.

关 键 词：应力 牙周膜成纤维细胞 细胞骨架 体外研究 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R780.2[医药卫生—基础医学]