Cloning of Partial Sodium Channel Gene From Strains of Fenvalerate-Resistant and Susceptible Cotton Aphid(Aphis gossypii Glover)  

作　　者：SUN Lu-juan, GAO Xi-wu and ZHENG Bing-zong(Department of Entomology , China Agricultural University, Beijing 100094 , P.R.China) 

出　　处：《Agricultural Sciences in China》2003年第8期881-885,共5页中国农业科学（英文版）

基　　金：supported by the National 973 Program(G2000016207);National 863 Program,China(2001AA249041).

摘　　要：The strain of fenvalerate-resistant cotton aphids was selected using fenvalerate insecticide in the laboratory, the resistance factor of the strain was 199.54. Three degenerate primers were designed and used to perform PCR amplification. A cDNA encoding partial sodium channel gene was cloned from the fenvalerate-resistant and -susceptible strains. There were two nucleotide acid differences between fenvalerate-resistant strain and -susceptible strain, resulting in an amino acid mutation, Met→Leu. It is predicted that the mutation is related to the cotton aphid resistance to fenvalerate.The strain of fenvalerate-resistant cotton aphids was selected using fenvalerate insecticide in the laboratory, the resistance factor of the strain was 199.54. Three degenerate primers were designed and used to perform PCR amplification. A cDNA encoding partial sodium channel gene was cloned from the fenvalerate-resistant and -susceptible strains. There were two nucleotide acid differences between fenvalerate-resistant strain and -susceptible strain, resulting in an amino acid mutation, Met→Leu. It is predicted that the mutation is related to the cotton aphid resistance to fenvalerate.

关 键 词：Cotton aphid Fenvalerate resistance Sodium channel 

分 类 号：S435.62[农业科学—农业昆虫与害虫防治]