胆汁泡蛋白酶联免疫吸附检测法的建立与初步临床应用  

ELISA kit for detection of biliary vesicular protein and its preliminary clinical application

作　　者：蔡端[1] 项建斌[1] 张延龄[1] 马保金[1] 

机构地区：[1]复旦大学附属华山医院外科,上海200040

出　　处：《中西医结合学报》2003年第3期218-220,共3页Journal of Chinese Integrative Medicine

基　　金：国家自然科学基金资助项目( 30 0 70 737);卫生部科研基金资助项目 ( 98 2 1 2 4 )

摘　　要：目的　建立胆汁泡蛋白快速检测法 ,作为防治胆石症临床研究的指标。方法　提取 3 3 .5kd胆汁泡蛋白 ,建立酶联免疫吸附检测法 (ELISA)标准曲线 ,并应用ELISA药盒测定正常人、胆石症患者胆汁和血清中泡蛋白含量 ,同时观察利胆冲剂、胆通胶囊等对泡蛋白的影响。结果　ELISA曲线方程为Y =0 .0 3 5X(r=0 .99) ;胆固醇性结石组胆囊胆汁和血清 3 3 .5kd泡蛋白含量分别为 (2 13 .4± 70 .1)mg/L、(179.8± 97.9)mg/L ,明显高于胆色素性结石症、非胆石症胆道疾病组以及正常人组 (P <0 .0 5) ,而后三者之间没有显著差异 (P >0 .0 5) ;利胆冲剂治疗两周后 ,胆汁和血清泡蛋白含量显著降低 ,而对照组和胆通胶囊治疗组未见明显变化。结论　胆汁泡蛋白的含量在不同人群差异显著 ;利胆冲剂等药物能降低胆汁和血清 3 3 .5kd泡蛋白含量 。ObjectiveTo establish a rapid and precise detectiv e method of 33.5 kd vesicular protein and to screen an effective treatment of chol elithiasis. MethodsSpecific antibody of the biliary vesicular prot ein was obtained by immunizing rabbits and enzyme-linked immunosorbent assay (E LISA) kit was developed. The concentrations of 33.5 kd vesicular protein in seru m and bile of gallstone patients and control were examined respectively. The eff ects of Cholagogue Dry Syrup and Eulektrol Capsule on decreasing 33.5 kd vesicul ar protein were also studied by ELISA kit. ResultsOne-step ELISA equation was Y= 0.035 X (r=0.99).The vesicular protein concentrations in seru m and bile of cholesterol gallstone group (179.8±97.9 ) mg/L and (213.4±70. 1)mg/L respectively were significantly (P <0.05) higher than in the pigme nt stone group and control. Data showed that, with 2-week administration, Chola gogue Dry Syrup significantly decreased both biliary and serum 33.5 kd vesicular protein of cholesterol gallstone patients, while Eulekrol Capsule and control g roups didn’t have the same results. Conclusion The concentrations o f 33.5 kd protein are different in cholesterol gallstone patients and healthy gr oups which might be related to cholesterol nucleation process. Cholagogue Dry Sy rup is of cholagogic and litholytic effect by decreasing biliary lithogenesis.

关 键 词：胆汁泡蛋白 酶联免疫吸附检测法 临床应用 利胆冲剂 

分 类 号：R446.6[医药卫生—诊断学]