枸杞多糖对氧化损伤大鼠晶状体上皮细胞凋亡相关基因Bcl-2和Bax表达的调控  

The regulation of LBP (lycium barbarum polysaccharides, LBP) on the expression of apoptosis-related genes Bcl-2 and Bax in SD rat LEC(lens epithelial cell, LEC) induced by oxidative injuries

作　　者：汪朝阳[1] 黄秀榕[1] 祁明信[2] 王勇[1] 

机构地区：[1]福建中医院现代医学部,福建福州350001 [2]福建省第二人民医院眼科,福建福州350003

出　　处：《眼视光学杂志》2003年第3期147-149,共3页Chinese Journal of Optometry & Ophthalmology

基　　金：国家中医药管理局重点课题(97Z403)

摘　　要：目的:研究枸杞多糖(lycium barbarum polysaccha-rides,LBP)对实验性氧化损伤大鼠晶状体上皮细胞(lens ep- ithelial cell,LEC)凋亡相关基因Bcl-2和Bax表达的调控。方法:复制大鼠晶状体氧化损伤模型,同时加入终浓度为1g/L的枸杞多糖共同孵育24h后,取晶状体前囊膜采用免疫组化法检测凋亡相关基因Bcl-2和Bax的蛋白表达。结果:Bcl-2和Bax在正常SD大鼠LEC中均有表达,Bcl-2的表达远较Bax表达强。H_2O_2组Bcl-2表达显著下调,Bax表达显著上调(Ridit检验,R_1值分别为0.97和0,P<0.01,差异均有非常显著性),Bcl-2/Bax比率下降。枸杞多糖可明显上调Bcl-2表达,下调Bax的表达(与H_2O_2组比较,P值均<0.01,差异有非常显著性),Bcl-2/Bax比率上升;其改变幅度与白内停组相比差异均有显著性(P值均<0.05)。结论:氧化损伤诱导LEC凋亡和枸杞多糖抑制LEC凋亡的分子机制可能与调控凋亡相关基因Bcl-2和Bax的表达有关。Objective: To investigate the effect of LBP on the expression of apoptosis-related genes of LEG that was induced by experimental oxidative injuries. Methods: All fresh transparent lenses of SD rats except in the control group were bathed in a culture medium with 300μmol/l hydrogen peroxide (H2O2) to establish cataract models in vitro. Meanwhile, herbs were added to the culture medium. Lenses were incubated for 24 hours. The anterior capsule films were used in the following study. The expressions of apoptosis-relat-ed genes Bcl-2/Bax protein were studied by immunohistochemical technique. Results: Bcl-2 and Bax protein were expressed in LEGs of normal SD rat lenses. Expression of Bcl-2 protein was much stronger than that of Bax protein. The expression of Bcl-2 protein was down-regulated and that of Bax protein was up-regilated in the H2O2 control group when compared to the control group(radit test,R equal 0.97 and 0,respectively, P< 0.01) so that the ratio of Bcl-2/Bax was lower. LBP up-regulated the expression of Bcl-2, and down-regulated that of Bax protein(in contrast to the H2O2 control group, P < 0.01). The ratio of Bcl-2/Bax was higher. There were large differences in the range of ratio changes compared to that of the pirenoxine sodium group( P <0. 01). Conclusion: LBP can up-regulate the expression of Bcl-2, and down-regulate that of Bax protein, increasing the ratio of Bcl-2/Bax;while the expression of Bcl-2 protein was down-regulated and that of Bax protein was up-regulated in the H2O2 control group. The molecular mechanisms of both H2O2 inducing apoptosis of LEC and natural anti-oxidants restraining apoptosis of LEC may be related to regulating the expression of apoptosis-related genes Bcl-2/Bax.

关 键 词：枸杞多糖 氧化损伤 大鼠 晶状体上皮细胞 细胞凋亡 基因表达 BCL-2 BAX 

分 类 号：R285[医药卫生—中药学]