中药南五味子及其混淆品绿叶五味子果实的ITS序列分析  

Ribosomal DNA ITS Sequences Analysis of the Chinese Crude Drug Fructus Schisandrae Sphenantherae and Fruts of Schisandra viridis

作　　者：高建平[1] 王彦涵[1] 乔春峰[1] 陈道峰[1] 

机构地区：[1]复旦大学药学院生药学教研室,上海200032

出　　处：《中国中药杂志》2003年第8期706-710,共5页China Journal of Chinese Materia Medica

基　　金：高校博士点基金项目 ( 2 0 0 0 0 2 6 519);高校优秀青年教师教学和科研奖励基金项目;国家自然科学基金项目( 30 2 71586 )

摘　　要：目的 :比较研究不同产地南五味子的基源植物华中五味子及混淆品绿叶五味子的rDNAITS碱基序列的差异及其规律 ,寻找南五味子和绿叶五味子果实之间的分子鉴别方法。方法 :PCR直接测序 ,PAUP 4 .0b1 0软件进行对位排列分析 ,采用邻接法 (neighbor joiningmethod)构建邻接 (NJ )系统树。 结果 :华中五味子ITS长度范围为 691～ 692bp ,ITS1和ITS2分别为 2 82bp和 2 4 6～ 2 4 7bp ;绿叶五味子ITS长度范围为 694～ 695bp ,ITS1和ITS2分别为 2 85～ 2 86bp和 2 4 6～ 2 4 7bp。不同产地华中五味子与绿叶五味子在ITS1有 3个稳定的信息位点。NJ树中 ,产于鸡公山 3个居群的绿叶五味子和天目山的 1个居群及引用的 2条绿叶五味子ITS序列聚为一支 ,bootstrap支持率为 68%。 结论Objective: To find the patterns of the rDNA ITS sequence variation of Schisandra sphenanthera and S.viridis , and to establish the molecular biological method for the identification of Fructus Schisandrae Sphenantherae and the fruits of S.viridis . Method: PCR products were sequenced directly and the sequences were analyzed with PAUP 4.0b10. NJ systematic tree was obtained with neighbor joining method. Result: The Complete ITS sequence of S. sphenanthera was 691 692 bp, of which there were 282 bp of ITS1 and 246 247 bp of ITS2. The complete sequence of S. viridis was 694 695 bp, consisting of 285 286 bp of ITS1 and 246 247 bp of ITS2. There were three informative sites in ITS1 regions for the two species. In the NJ tree with Kadsura anamosma and K. coccinea as outgroups, five different populations of S. viridis were the monophyletic group with the bootstrap value of 68%. These populations included one from Tianmushan, Zhejiang province, three populations from Jigongshan, Henan Province and the other two populations of S. viridis cited the sequences from GeneBank (registration numbers are AF263438 and AF163703 respectively). Conclusion: The rDNA internal transcribed spacer is a good marker to distinguish the Fructus Schisandrae Sphenantherae from the fruits of S. viridis.

关 键 词：南五味子 绿叶五味子 DNA碱基序列 药材鉴别 

分 类 号：R282.5[医药卫生—中药学]