RP-HPLC测定六味地黄方中苷类成分的含量  

Determination of Glycosides in Traditional Chinese Medicine Liu-wei Di-huang by RP-HPLC

作　　者：熊善丽[1] 赵毅民[1] 栾新慧[1] 乔善义[1] 孙磊[1] 任凤霞[1] 郭继芬[1] 张永祥[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《中国中药杂志》2003年第8期735-738,共4页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究发展计划 ( 973)项目 (G19990 54 40 1)

摘　　要：目的 :建立RP HPLC测定六味地黄方中苷类成分含量的方法。方法 :选用C1 8色谱柱 ,甲醇 水 (33∶67)为流动相 ,检测波长 2 36nm ,柱温 30℃下进行含量测定。结果 :六味地黄方水煎液中莫诺苷、马钱素、芍药苷进样浓度分别在 7.4～ 60 ,7.7～ 62 ,8.5～ 68mg·L- 1 与其色谱峰面积呈良好线性关系 ,加样回收率分别为 98.8% ,98.3 % ,99.6 %。结论 :本方法简便、快速、准确 。Objective: To establish a RP HPLC method for determination of glycosides in Traditional Chinese Medicine Liu wei Di huang. Method: The samples were analyzed on an ODS column at 30 ℃, with mobile phase of methanol/water (33∶67) at flow rate 1.0 mL·min and detection at wavelength of 236 nm. Result: Three major components reached base line separation and were identified to be mononiside, loganin, paeoniflorin. Respectively for the three components, linear correlations were found between peak areas and concentrations in the ranges of 7.4～60,7.7～62 mg·L -1 and 8.5～68 mg·L -1 , and the recoveries were 98.8%, 98.3%, 99.6%. Conclusion: The established method is proved to be suitable for simultaneous quantification of three major glycosidic components in Liuwei Dihuang decoction and can be used for evaluation of the quality of Liuwei Dihuang preparations.

关 键 词：六味地黄方 苷类成分 含量测定 反相高效液相色谱法 

分 类 号：R289.5[医药卫生—方剂学]