鸦胆子油乳诱导HL-60细胞凋亡的研究  

Experimental Studies on the Apoptosis of HL-60 Cells Induced by Brucea Javanica Oil Emulsion

作　　者：王芳[1] 曹玉[1] 刘红岩[1] 付招娣[1] 韩锐[1] 

机构地区：[1]中国医学科学院药物研究所,北京100050

出　　处：《中国中药杂志》2003年第8期759-762,共4页China Journal of Chinese Materia Medica

摘　　要：目的 :研究鸦胆子油乳诱导HL 60细胞凋亡的作用。方法 :鸦胆子油乳 1∶1 0 0 ,1∶40 ,1∶2 0 (V/V)分别处理HL 60细胞 6h ,琼脂糖凝胶电泳法 ,流式细胞仪法 ,荧光显微镜法 ,电镜分别观察药物诱导HL 60细胞凋亡的作用。结果 :鸦胆子油乳 1∶40组产生明显DNA梯状条带 ;流式细胞仪检测药物浓度为 1∶40 ,1∶2 0组的凋亡细胞百分率分别为 86 .8%和 97% ;鸦胆子油乳 1∶40组在荧光显微镜呈现明显凋亡细胞特征 ;药物浓度为 1∶40 ,1∶2 0组的细胞经电镜观察呈现明显凋亡细胞特征。结论 :鸦胆子油乳 1∶2 0和 1∶40组有明显的诱导HLObjective:To investigate the inducing effect of Brucea javanica on the apoptpsis of HL 60 cells. Method: HL 60 cells were treated with Brucea javanica 1∶100,1∶40,1∶20 (v/v) respectively for 6 h and DNA agarose gel electrophoresis, flow cytometry, fluorescence microscope, electron microscope were used to observe the apoptosis inducing effect of Brucea javanica. Result: DNA ladder was seen in the 1∶40 group. The apoptosis cell percentages of 1∶40 and 1∶20 group were 86.8% and 97% respectively. Cells of 1∶40 group showed obvious apoptosis character under fluorescence microscope. Cells were induced apoptosis in 1∶20 and 1∶40 Brucea javanica under electron microscope. Conclusion: 1∶20 and 1∶40 Brucea Javanica showed obvious apoptosis inducing effect of HL 60.

关 键 词：鸦胆子油乳 白血病 HL-60细胞 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]