多波长系数法测定痤疮露Ⅲ号中3种成分的含量  

Multiwavelength Coefficient Method for Determination of the Contents of Three Ingredients in Acne Ⅲ

作　　者：孙燕[1] 李凯[2] 刘璟[1] 王芳[1] 韩军[1] 

机构地区：[1]兰州军区乌鲁木齐总医院药剂科,乌鲁木齐市830000 [2]第一军医大学中药系,广州市510515

出　　处：《中国药房》2003年第9期556-557,共2页China Pharmacy

摘　　要：目的:建立检测痤疮露Ⅲ号中3种成分含量的方法。方法:在紫外分光光度法的基础上,建立多波长系数法测定替硝唑、氯霉素、水杨酸的含量。结果:本方法可同时测定3种成分的含量。替硝唑、氯霉素及水杨酸的线性范围分别为3.96～11.88μg/ml(r=0.9 992)、4.12～12.36μg/ml(r=0.9 993)、4.0～12.0μg/ml(r=0.9 997);平均回收率分别为 98.39％、100.9％、100.4％;精密度分别为1.53％、1.09％、1.17％。结论:本方法用于测定光谱重叠严重的多种成分含量,方法简便、快捷,结果准确,适合医院制剂质量控制的需要。To establish a multiwavelength coefficient method to determine the three components in Acne Ⅲ. METHODS: On the basis of UV -- spectrophotometry, multiwavelength coefficient method was developed to simultaneously determine the contents of tinidazole, chloramphenicol and salicylic acid .RESULTS: The calibrating curves of tinidazole, chlo- ramphenicol and salicylic acid were linear in the ranges of 3.96～11.88μg/ ml(r=0.9 992), 4.12～12.36μg/ml (r=0.9 993) and 4.0～12.0μg/ml(r=0.9 997) with average recoveries of 98.39% (RSD=1.53%), 100.9% (RSD=1.09%) and 100.4% (RSD=1.17%), respectively .CONCLUSION: The method is simple, accurate and can be used for determination of the con- tents of multi -- components with overlapping spectra, and it is suitable for the quality control of hospital preparations.

关 键 词：替硝唑 氯霉素 水杨酸 多波长系数法 

分 类 号：R927.2[医药卫生—药学]