周围神经损伤后内源性碱性成纤维细胞生长因子的表达  

Experimental Study on Endogenous Basic Fibroblast Growth Factor Expression after Injury of Peripheral Nerve

作　　者：张朝[1] 郑玉云[1] 陈瑞[1] 

机构地区：[1]内蒙古包头医学院第一附属医院骨科,014010

出　　处：《中国误诊学杂志》2003年第9期1294-1296,共3页Chinese Journal of Misdiagnostics

基　　金：内蒙古包头医药卫生发展基金资助项目 (99- 2 7)

摘　　要：目的 :探讨周围神经损伤后内源性碱性成纤维细胞生长因子 (b FGF)的变化规律及与神经修复的关系。方法 :5 6只 Wistar大鼠 ,随机分为坐骨神经钳夹组 (n=4 9)和正常组 (n=7)。采用免疫组化、计算机图像处理技术 ,对正常和损伤后不同时间的坐骨神经中 b FGF的表达进行检测。结果 :1采用钳夹的方式可使坐骨神经 b FGF表达增加。 2神经损伤后 4 h局部 b FGF开始升高 ,7～ 14 d达峰值 ,2 1d开始下降 ,2 8d恢复正常。结论 :1周围神经损伤后 b FGF表达变化与伤后时间有着时效关系。 2周围神经损伤后 b FGF表达明显增高 ,可能介导了神经损伤后一系列的细胞反应 ,以间接和直接的方式促进了周围神经的再生。Objective:To explore the change of endogenous basic fibroblast growth factor(bFGF) in the course of injury and regeneration of peripheral nerve. Methods:56 wistar rats were divided randomly into crushing group (n=49) and normal group(n=7).In the crushing group,The expression of endogenous basic fibroblast growth factor of normal and injury nerves were examined by immunohistochemical technique and image analysis.Results:① In crushing group ,the expression of bFGF was markedly higher than that in the control and normal group.②At 4 hours after crushing bFGF was raised , reached peak 7～14 days later, and recovered 28th day later.Conclusions:After injury of peripheral nerve, the presence and up-regulation of bFGF may be involved in regengration of peripheral nerve system.

关 键 词：周围神经/损伤 成纤维细胞生长因子2/代谢 神经再生 

分 类 号：R338[医药卫生—人体生理学]