低锌和缺锌对玉米生长发育的影响  

Effect of Low Zinc and Zinc Deficiency on Maize Growth

作　　者：房蓓[1] 武泰存[1] 王景安[1] 

机构地区：[1]天津师范大学化学与生命科学学院,中国天津300074

出　　处：《生命科学研究》2003年第3期255-261,共7页Life Science Research

基　　金：天津师范大学引进人才基金项目(5RL002)

摘　　要：以玉米沈单10号为材料,用溶液培养的方法研究了缺锌、低锌和正常供锌对玉米生长发育的影响,进一步明确了一定量低锌比缺锌对玉米的伤害更大,并对其POD、SOD、CAT同工酶谱及蛋白质表达进行了分析.结果表明:一定浓度的低锌培养使玉米生长受抑及受害最重,且地上部分比地下部分更敏感.低锌和缺锌处理时3种同工酶的酶谱和蛋白表达与正常供锌时均有明显的差异,尤其在一定量低锌浓度时,有些同工酶的表达增强或被特异性诱导,而另一些酶的合成受阻;低锌和缺锌处理都诱导出了新的蛋白组分,也缺失了部分蛋白组分,且低锌比缺锌时缺失的蛋白组分更多,这些变化可能与玉米生长发育及受害密切相关.Compared to optimal Zn supply, certain concentration of low Zinc was found to injure maize (Zea mays L.) growth more severely than Zn deficiency treatment in solution culture experiment on Shendan10 (SD10). A further analysis on POD (peroxidase), SOD (superoxide dismutase), CAT (catalase) isozymes, and protein expression in SDSPAGE was discussed in this discourse and conclusions are drawn as follows: plants grown in the low Zinc nutrition solution (about 0.001 μmol/L) were stunted severest, and more visible deficiency symptoms appeared in shoot than that in root. The above three isozyme and protein expressions presented obvious difference under low Zn and Zn deficiency treatment from that at optimal Zn supply. At certain low Zn, some isozyme expressions were enhanced or specifically induced while some were inhibited. Under low Zn and Zn deficiency treatment, some new protein components occurred, while some original components were lost. However, low Zn caused more protein components disappeared. These changes may have a close relationship with maize growth and injury.

关 键 词：低锌 缺锌 玉米 生长发育 同工酶 蛋白质电泳 

分 类 号：S513[农业科学—作物学]