荧光偏振法测定荧光素钠荧光寿命的研究  

STUDY OF FLUORESCENCE POLARIIMETRIC METHOD FOR DETERMINATION OF SODIUM FLUORESCEIN LIFETIME

作　　者：张宝棣[1] 王继波[1] 刘玉兰[1] 

机构地区：[1]青岛医学院化学教研室

出　　处：《青岛医学院学报》1992年第2期114-117,共4页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：用荧光偏振法测定荧光素钠荧光寿命的灵敏度和准确度较高.在荧光素钠荧光强度最强时——激发波为490nm,发射波为515nm,pH值8.5,溶液浓度1.6×10^(-5)mol/L,测定荧光素钠溶液的荧光偏振度,测定不同温度时荧光素钠溶液的粘度,再根据Perrin公式,求出荧光素钠的荧光寿命.用荧光偏振法测得荧光素钠荧光寿命为3.4ns.A simple, fast and highly accurate method for determining the lifetime of Sodium fluorescein is presented.Under the condition of λex=490nm, λem=515nm, C=1.6×10 ~5mol/L and pH=8.5, we measured the flucrescent polarity of sodium floerescein solution by using flucrescence polarimetric methed. The sodium fluorescein was lifetime calculated according to Perrin formula was 3.4ns.

关 键 词：荧光偏振法 荧光素钠 荧光寿命 

分 类 号：R446[医药卫生—诊断学]