乌头注射液对辐射损伤小鼠胸腺细胞的作用  

Effect of Aconite Injection on the Spontaneous Incorporation of 3H-TdR into Thymocytes in the Mice of Radiation Damage

作　　者：孟庆勇[1] 刘志辉[1] 徐美奕[1] 高莉莉[1] 

机构地区：[1]广东医学院分析中心,广东湛江524023

出　　处：《中国辐射卫生》2003年第3期139-140,共2页Chinese Journal of Radiological Health

基　　金：广东省中医药管理局科研基金资助 (No:9841 9)

摘　　要：目的　观察乌头注射液对辐射损伤小鼠胸腺细胞自发掺入3 H -TdR的作用。方法　检测小鼠胸腺细胞自发掺入3 H -TdR的百分率。结果　体外实验显示实验组 (终浓度为 2× 10 -11μg/ml和 4× 10 -9μg/ml乌头注射液 )的小鼠胸腺细胞自发掺入3 H -TdR的百分率明显高于对照组。体内实验表明 1μg/kg的乌头注射液加照射组的小鼠胸腺细胞自发掺入3 H -TdR的百分率比单纯照射组明显增加。结论　低浓度的乌头注射液对体外小鼠胸腺细胞自发掺入3 H -TdR具有明显的刺激作用 ,而适宜剂量的体内用药对辐射损伤小鼠胸腺细胞自发掺入3 HObjective To observe the effect of aconite in jection on the spontaneous incorporation of 3H-TdR into thymocytes in the mice of radiation damage.Methods The spontaneous incorporation of 3H-TdR into thymocytes was examined.Results Experiment in vitro shows that the percentage of the spontaneous incorporation of 3H-TdR into thymocytes in aconite injection(2×10 -11 μg/mL and 4×10 -9 μg/mL)group is higher than that of the control group.The percentage in aconite injection(1 μg/kg)with radiation group is markedly than that in control group in the mice of radiation damage in vivo.Conclusion Aconite injection had the stimulating effect in the spontaneous incorporation of 3H-TdR into thymocytes in vitro and aconite injection of proper dosage can enhance the spontaneous incorporation radiation damage mice in vivo.

关 键 词：乌头注射液 辐射损伤 小鼠 胸腺细胞 自发掺入 自由基 

分 类 号：R818.84[医药卫生—放射医学] R285.5[医药卫生—临床医学]