新型多吡啶配体的设计及其对配合物与DNA作用机制的影响  

Design of New Polypyridyl Ligands and Their Effects on DNA-binding Mechanisms of Complexes

作　　者：张黔玲[1] 刘剑洪[1] 刘建忠[2] 任祥忠[1] 张培新[1] 魏波[1] 徐宏[1] 计亮年[2] 

机构地区：[1]深圳大学师范学院化学与生物学系,深圳518060 [2]中山大学化学与化工学院,广州510275

出　　处：《高等学校化学学报》2003年第10期1753-1755,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :2 980 10 0 5 )资助

摘　　要：Two structurally related polypyridyl ligands ODHIP(3,4-dihydroxyl-imidazophenanthroline), MDHIP(2,4-dihydroxyl-imidazo phenanthroline) and their ruthenium(II) complexes [Ru(phen) 2ODHIP] 2+ and [Ru(phen) 2MDHIP] 2+ were prepared and characterized. Their DNA-binding properties were studied by spectroscopic methods and viscosity measurements. The results indicated that the two complexes are bound to DNA by different modes due to the different planarities of ligands. For the complex [Ru(phen) 2MDHIP] 2+, the 2- position ortho group of MDHIP could form an intramolecular hydrogen bond with the nitrogen atom of the imidazole ring to extend the planarity and strengthen the binding affinity. On the other hand, the 4- position ortho group hindered the complex from intercalating into the base pairs of DNA, and finally, making the complex bind to DNA by a partial intercalative mode. However, for the complex [Ru(phen) 2ODHIP] 2+, there was no intramolecular hydrogen bond formed and the two ortho groups further increased the steric effect and decreased the binding affinity, thus making the complex bind to DNA by groove binding mode.Two structurally related polypyridyl ligands ODHIP(3,4-dihydroxyl-imidazophenanthroline), MDHIP(2,4-dihydroxyl-imidazo phenanthroline) and their ruthenium(II) complexes [Ru(phen) 2ODHIP] 2+ and [Ru(phen) 2MDHIP] 2+ were prepared and characterized. Their DNA-binding properties were studied by spectroscopic methods and viscosity measurements. The results indicated that the two complexes are bound to DNA by different modes due to the different planarities of ligands. For the complex [Ru(phen) 2MDHIP] 2+, the 2- position ortho group of MDHIP could form an intramolecular hydrogen bond with the nitrogen atom of the imidazole ring to extend the planarity and strengthen the binding affinity. On the other hand, the 4- position ortho group hindered the complex from intercalating into the base pairs of DNA, and finally, making the complex bind to DNA by a partial intercalative mode. However, for the complex [Ru(phen) 2ODHIP] 2+, there was no intramolecular hydrogen bond formed and the two ortho groups further increased the steric effect and decreased the binding affinity, thus making the complex bind to DNA by groove binding mode.

关 键 词：多吡啶钌配合物 设计 DNA 结构识别 作用机制 插入作用 沟面作用 

分 类 号：Q523[生物学—生物化学] Q503