SPR免疫竞争法研究小分子吗啡与其抗体间作用的动力学特征  

Kinetic Characteristics of the Interaction Between Morphine and Its Antibody by SPR Competitive Immunoassay

作　　者：陈受惠[1] 崔小强[1] 杨帆[1] 倪永年[2] 杨秀荣[1] 

机构地区：[1]中国科学院长春应用化学研究所电分析化学国家重点实验室,长春130022 [2]南昌大学化学系,南昌330047

出　　处：《高等学校化学学报》2003年第10期1770-1774,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :2 0 0 75 0 2 7);国家重点基础研究规划项目 (批准号 :2 0 0 1CB5 10 2 )资助

摘　　要：表面等离子体共振技术 ( SPR)主要应用于生物大分子相互作用的研究 ,本文采用溶液竞争法 ,测定了小分子吗啡与其抗体作用的结合常数 K1,并计算了吗啡抗体与吗啡化牛血清白蛋白的结合常数 K2 .证明了多抗对抗原的亲和力较单抗大 ,并表明大分子蛋白质的存在对抗体与待测物的结合有阻碍作用 .It is impossible for surface plasmon resonance to measure directly the binding kinetics between a low-molecular-weight analyte interacting and its immobilized binding partner. Solution competition method was applied to the kinetic study of the interaction between morphine and its antibody. The affinity constant between the antibody of morphine and morphine-BSA immobilized on the sensor chip was also obtained. The result showed that the affinity of polyclonal antibody is stronger than that of monoclonal antibody. And it also indicated that the protein combined with the analyte affected the binding of antibody to antigen.

关 键 词：表面等离子体共振 动力学 吗啡 溶液竞争法 免疫分析 抗体 止痛剂 

分 类 号：R971.2[医药卫生—药品] R966[医药卫生—药学]